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Phenotypic divergence of thermotolerance: Molecular basis and cold adaptive evolution related to intrinsic DNA flexibility of glacier-inhabiting Cryobacterium strains.
Environmental Microbiology ( IF 4.3 ) Pub Date : 2020-02-28 , DOI: 10.1111/1462-2920.14957
Qing Liu 1 , Wei-Zhi Song 2 , Yu-Guang Zhou 1 , Xiu-Zhu Dong 3 , Yu-Hua Xin 1
Affiliation  

The link between guanine-cytosine (GC) content and thermal adaptation is controversial. Here, we compared maximum growth temperature (TMGT ) and genomics of 78 Cryobacterium strains to avoid unreliable conclusions resulting from distantly phylogenetic groups. Phylogenomic analysis revealed this taxon had much higher diversification than we knew. Interestingly, these strains showed thermotolerance divergence with phylogenetic cohesion. A significant difference was found between TMGT ≤ 20°C strains and TMGT > 20°C strains in genomic GC content which mainly caused by variation of GC3. TMGT ≤ 20°C strains tended to use synonymous codons ended with A/U, but TMGT > 20°C strains tended to use G/C. Lower GC content at synonymous sites (≈GC3) of TMGT ≤ 20°C strains could provide lower intrinsic DNA flexibility which strongly associated with optimal molecular dynamics, and then guarantee DNA function at lower growth temperatures. This analysis of codon bias revealed close relationships for thermal adaptation, GC content at synonymous sites (≈GC3), intrinsic DNA flexibility and optimal DNA dynamics. Natural selection was main force driving this codon bias; strains with lower TMGT endured stronger natural selection. Therefore, this study provided molecular basis for bacterial adaptive evolution from moderate temperature to low temperature.

中文翻译:

表型差异的耐热性:冰川基础的低温细菌菌株固有的DNA柔韧性相关的分子基础和冷适应进化。

鸟嘌呤-胞嘧啶(GC)含量与热适应之间的联系是有争议的。在这里,我们比较了78株低温细菌的最高生长温度(TMGT)和基因组学,以避免由于遥远的系统发生群体而得出的不可靠结论。系统学分析表明,该分类单元比我们所知道的具有更高的多样性。有趣的是,这些菌株表现出耐热性差异和系统发生内聚力。发现TMGT≤20°C菌株与TMGT> 20°C菌株之间的基因组GC含量存在显着差异,这主要是由GC3的变化引起的。TMGT≤20°C菌株倾向于使用以A / U结尾的同义密码子,而TMGT> 20°C菌株倾向于使用G / C。TMGT≤20°C菌株的同义位点(≈GC3)较低的GC含量可提供较低的固有DNA柔韧性,这与最佳分子动力学密切相关,然后可确保较低生长温度下的DNA功能。密码子偏倚的分析揭示了热适应,同义位点的GC含量(≈GC3),固有的DNA柔韧性和最佳的DNA动力学密切相关。自然选择是推动这种密码子偏向的主要力量。TMGT较低的菌株可承受更强的自然选择。因此,本研究为细菌从中温到低温的适应性进化提供了分子基础。固有的DNA灵活性和最佳的DNA动力学。自然选择是推动这种密码子偏向的主要力量。TMGT较低的菌株可承受更强的自然选择。因此,本研究为细菌从中温到低温的适应性进化提供了分子基础。固有的DNA灵活性和最佳的DNA动力学。自然选择是推动这种密码子偏向的主要力量。TMGT较低的菌株可承受更强的自然选择。因此,本研究为细菌从中温到低温的适应性进化提供了分子基础。
更新日期:2020-04-01
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