Development of a Droplet Microfluidics Device Based on Integrated Soft Magnets and Fluidic Capacitor for Passive Extraction and Redispersion of Functionalized Magnetic Particles,Advanced Materials Technologies

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Development of a Droplet Microfluidics Device Based on Integrated Soft Magnets and Fluidic Capacitor for Passive Extraction and Redispersion of Functionalized Magnetic Particles
Advanced Materials Technologies ( IF 6.4 ) Pub Date : 2020-02-24 , DOI: 10.1002/admt.201901088
Marco Serra _{1,

2} , Emilie Gontran _{1,

2} , Ismail Hajji _{1,

2} , Laurent Malaquin _{1,

2,

3} , Jean‐Louis Viovy _{1,

2} , Stephanie Descroix _{1,

2} , Davide Ferraro _{1,

2,

4}

Affiliation

Based on a recent droplet microfluidic device for target molecules' extraction, purification, and redispersion, here an original fluidic control system is proposed to improve the device versatility and usability. This approach relies on the coupling of functionalized magnetic particles handling by integrated soft magnets and a passive droplet splitting control at a channel junction. To reach the high standards required for bioassays' implementation, this device integrates a pressure‐driven variable volume component, acting as a fluidic capacitor, which allows a passive and controlled self‐triggered droplet fingering in a dead‐end channel. The working principle is described, and the modeling of the induced fingering phenomenon by an electric analogy is presented. Then, the achieved finger length is characterized as a function of geometrical and material properties of the fluidic capacitor. The complete device configuration guarantees extraction and purification efficiencies higher than 97% and 95%, respectively, showing high‐throughput particles handling between droplets with a passive droplet splitting control. Finally, this technology is successfully applied to gene expression study for cancer diagnosis by performing magnetic particle–based messenger RNA (mRNA) extraction followed by reverse transcription (RT) and quantitative polymerase chain reaction (RT‐qPCR) analysis.

中文翻译：

基于集成软磁铁和流体电容器的液滴微流控装置的开发，用于功能性磁性粒子的被动萃取和再分散

基于最近用于目标分子的提取，纯化和再分散的液滴微流控设备，在此提出一种原始的流体控制系统，以提高设备的多功能性和可用性。这种方法依赖于通过集成软磁铁和通道结处的无源液滴分裂控制对功能化磁性颗粒进行处理的耦合。为了达到实施生物测定所需的高标准，该设备集成了一个压力驱动的可变体积组件，用作流体电容器，允许在死端通道中进行无源和可控的自触发液滴指法。描述了工作原理，并通过电气类比对感应指法现象进行了建模。然后，所获得的指状长度的特征在于流体电容器的几何和材料特性。完整的设备配置确保提取和纯化效率分别高于97％和95％，显示了通过被动液滴分裂控制实现液滴之间高通量颗粒的处理。最后，这项技术通过执行基于磁性粒子的信使RNA（mRNA）提取，然后进行逆转录（RT）和定量聚合酶链反应（RT-qPCR）分析，成功应用于癌症的基因表达研究。显示了通过被动液滴分裂控制在液滴之间处理高通量颗粒的过程。最后，这项技术通过执行基于磁性粒子的信使RNA（mRNA）提取，然后进行逆转录（RT）和定量聚合酶链反应（RT-qPCR）分析，成功应用于癌症的基因表达研究。显示了通过被动液滴分裂控制在液滴之间处理高通量颗粒的过程。最后，该技术通过执行基于磁性粒子的信使RNA（mRNA）提取，然后进行逆转录（RT）和定量聚合酶链反应（RT-qPCR）分析，成功应用于癌症的基因表达研究。

更新日期：2020-02-24

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