Deformities in human soft tissue caused by trauma or burn present a difficult problem in plastic surgery. In this study, we encapsulated troglitazone and angiotensin 1-7 mimetic AVE0991 in gelation microspheres with the goal of inducing epithelial transformation for potential applications in tissue reconstruction. After troglitazone or AVE0991 were encapsulated to gelation microspheres, their release kinetics and bioactivity were examined. Surface morphology and diameter of the gelation microspheres were evaluated using light microscopy. The release of the drugs was assessed in the presence of human adipose-derived stem cells (ADSCs). Treatment with troglitazone microspheres increased cell viability and activated the β-catenin in ADSCs. Moreover, the AVE0991 microspheres also increased cell viability and C-myc expression of ADSCs. These results showed that troglitazone and AVE0991 microspheres promoted the activity of ADSCs. Furthermore, ADSCs were co-treated with troglitazone and AVE0991 microspheres. Western blot and immunofluorescent staining showed that co-treatment with troglitazone and AVE0991 microspheres elevated the expression of epithelialization associated protein CK14 in ADSCs. In conclusion, our findings indicate that microspheres with troglitazone and AVE0991 can significantly improve the viability and epithelialization of ADSCs, which provides a new approach for the construction of tissue-engineered skin.

中文翻译：

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胶凝微球对曲格列酮和AVE0991的封装促进了脂肪干细胞的上皮转化。

由外伤或烧伤导致的人类软组织畸形是整形外科中的难题。在这项研究中，我们封装了曲格列酮和血管紧张素1-7模拟AVE0991在胶凝微球中，目的是诱导上皮转化，以潜在地用于组织重建。将曲格列酮或AVE0991封装到凝胶微球后，检查其释放动力学和生物活性。使用光学显微镜评估凝胶微球的表面形态和直径。在存在人类脂肪干细胞（ADSC）的情况下评估了药物的释放。曲格列酮微球治疗可提高细胞活力并激活ADSC中的β-连环蛋白。此外，AVE0991微球还增加了ADSCs的细胞活力和C-myc表达。这些结果表明曲格列酮和AVE0991微球促进了ADSC的活性。此外，ADSC与曲格列酮和AVE0991微球共同治疗。Western印迹和免疫荧光染色表明，曲格列酮和AVE0991微球共同处理可提高ADSC中上皮化相关蛋白CK14的表达。总之，我们的研究结果表明，曲格列酮和AVE0991的微球可以显着改善ADSC的活力和上皮形成，这为组织工程皮肤的构建提供了一种新方法。Western印迹和免疫荧光染色表明，曲格列酮和AVE0991微球共同处理可提高ADSCs上皮化相关蛋白CK14的表达。总之，我们的研究结果表明，曲格列酮和AVE0991的微球可以显着改善ADSC的活力和上皮形成，这为组织工程皮肤的构建提供了一种新方法。Western印迹和免疫荧光染色表明，曲格列酮和AVE0991微球共同处理可提高ADSCs上皮化相关蛋白CK14的表达。总之，我们的研究结果表明，曲格列酮和AVE0991的微球可以显着改善ADSC的活力和上皮形成，这为组织工程皮肤的构建提供了一种新方法。