Lignocellulosic biomass is the most abundant sustainable carbon source on the planet and has enormous potential to substitute fossil resources on the premise of cost-effective conversion. Efforts have been made to develop various lignocellulosic bioconversion strategies to overcome biomass recalcitrance, promote product conversion efficiency and reduce process cost. Consolidated bio-saccharification (CBS), a consolidated bioprocessing (CBP) derived strategy, is herein proposed for lignocellulose bioconversion by integrating enzyme production and hydrolysis steps but separating fermentation from the integrated process. This strategy employs cellulosome-producing microorganisms as a biocatalyst to enhance lignocellulose solubilization and produces lignocellulose-derived fermentable sugars as a platform product for fermentations aiming at various products. The success of CBS depends on robust biocatalysts with high activity, suitable pretreatments for efficient delignification, and downstream fermentations with process compatibility. The review introduces the updated progress on lignocellulose bioconversion following the CBS route, discusses key factors for optimization of the CBS process, and, more importantly, highlights challenges and promising solutions for the CBS strategy in the industrial application of lignocellulose bioconversion.

木质纤维素生物质是地球上最丰富的可持续碳源，在具有成本效益的转化前提下，具有替代化石资源的巨大潜力。已经做出努力来开发各种木质纤维素生物转化策略，以克服生物质的顽固性，提高产品转化效率并降低工艺成本。本文提出了整合的生物糖化（CBS），整合的生物加工（CBP）衍生的策略，其通过整合酶生产和水解步骤但将发酵与整合的过程分开来进行木质纤维素的生物转化。该策略采用产生纤维素的微生物作为生物催化剂来增强木质纤维素的增溶作用，并产生木质纤维素衍生的可发酵糖作为针对各种产品进行发酵的平台产品。CBS的成功取决于具有高活性的稳健生物催化剂，有效脱木素的合适预处理以及具有工艺兼容性的下游发酵。该评论介绍了遵循CBS路线进行木质纤维素生物转化的最新进展，讨论了优化CBS工艺的关键因素，更重要的是，重点介绍了木质纤维素生物转化在工业应用中CBS策略的挑战和有希望的解决方案。适用于高效脱木素的预处理，以及具有工艺兼容性的下游发酵。该评论介绍了遵循CBS路线进行木质纤维素生物转化的最新进展，讨论了优化CBS工艺的关键因素，更重要的是，重点介绍了木质纤维素生物转化在工业应用中CBS策略的挑战和有希望的解决方案。适用于高效脱木素的预处理，以及具有工艺兼容性的下游发酵。综述介绍了遵循CBS路线进行木质纤维素生物转化的最新进展，讨论了优化CBS工艺的关键因素，更重要的是，重点介绍了木质纤维素生物转化在工业应用中CBS策略的挑战和有希望的解决方案。