Targeted proteomics techniques allow accurate quantitative measurements of analytes in complex matrices with dynamic linear ranges that span up to 4–5 orders of magnitude. Hence, targeted methods are promising for the development of robust protein assays in several sensitive areas, for example, in health care. However, exploiting the full method potential requires reliable determination of the dynamic range along with related quantification limits for each analyte. Here, a software named CalibraCurve that enables an automated batch‐mode determination of dynamic linear ranges and quantification limits for both targeted proteomics and similar assays is presented. The software uses a variety of measures to assess the accuracy of the calibration, namely precision and trueness. Two different kinds of customizable graphs are created (calibration curves and response factor plots). The accuracy measures and the graphs offer an intuitive, detailed, and reliable opportunity to assess the quality of the model fit. Thus, CalibraCurve is deemed a highly useful and flexible tool to facilitate the development and control of reliable SRM/MRM‐MS‐based proteomics assays.

中文翻译：

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CalibraCurve：用于校准基于MS的目标测量的工具。

靶向蛋白质组学技术可对复杂基质中的分析物进行准确的定量测量，其动态线性范围可达4–5个数量级。因此，有针对性的方法有望在几个敏感领域，例如卫生保健领域中开发出强大的蛋白质测定方法。但是，要充分利用方法的潜力，就需要可靠地确定动态范围以及每种分析物的相关定量限。此处介绍了一种名为CalibraCurve的软件，该软件可针对目标蛋白质组学和类似检测方法，自动确定动态线性范围和定量限的批处理模式。该软件使用多种方法来评估校准的准确性，即准确性和真实性。将创建两种不同类型的可定制图形（校准曲线和响应因子图）。准确性度量和图形为评估模型拟合的质量提供了直观，详细且可靠的机会。因此，CalibraCurve被认为是一种非常有用且灵活的工具，可促进开发和控制基于SRM / MRM-MS的可靠蛋白质组学检测方法。