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Isolation of Trichoderma in the rhizosphere soil of Syringa oblata from Harbin and their biocontrol and growth promotion function.
Microbiological Research ( IF 6.7 ) Pub Date : 2020-02-22 , DOI: 10.1016/j.micres.2020.126445
Bin Liu 1 , Shida Ji 2 , Huifang Zhang 3 , Yucheng Wang 2 , Zhihua Liu 4
Affiliation  

For the effective biocontrol of Syringa powdery mildew (Mircosphaera syringejaponicae) and to promote seedling growth, we identified 44 of the 181 Trichoderma isolates (T1-T181) isolated from the rhizosphere soil. Analysis identified 10 Trichoderma species, and T. pseudoharzianum T1 (TpseT1), T. afroharzianum T52 (TafrT52), and T. asperelloides T57 (TaspT57) were selected to make Trichoderma biofertilizer because of their fast growth and high spore production. Exposing Syringa oblata to Trichoderma biofertilizer showed that TafrT52 and TaspT57 could induce abscisic acid (ABA) production, and promote the shedding of diseased leaves and the generation of new leaves. Furthermore, TafrT52 increased the catalase (CAT) activity and reduced the H2O2 content. And the disease incidence was reduced by 37.84 % by Tasp (highest) in 2017 year and by 13.84 % by TpseT1(lowest) in 2018 year. In addition, all Trichoderma strains we selected could promote the lateral root growth of S. oblata seedlings; however, because of the downregulated gene expression at the late stage of chlorophyll synthesis, the chlorophyll content decreased in the new leaves. Antagonism among different Trichoderma species led to low biocontrol and growth promotion effects, thus the Trichoderma mixture cannot be use as biofertilizer. TafrT52, with better biocontrol and growth promotion effects, could be used for biocontrol of M. syringejaponicae.



中文翻译:

哈尔滨紫丁香根际土壤中木霉的分离及其生防和促生作用。

为了有效控制丁香白粉病(Micosphaera printerjaponicae)并促进幼苗生长,我们从根际土壤中鉴定出181种木霉菌(T1-T181)中的44种。分析确定了10个木霉种,T. pseudoharzianum T1(TpseT1),T。afroharzianum T52(TafrT52)和T. asperelloides选择T57(TaspT57),使木霉,因为他们的快速增长和高孢子生产的生物肥料。将丁香丁香暴露于木霉生物肥料表明,TafrT52和TaspT57可以诱导脱落酸(ABA)的产生,并促进病叶脱落和新叶片的产生。此外,TafrT52增加了过氧化氢酶(CAT)活性并降低了H 2 O 2含量。Tasp(最高)在2017年减少了37.84%,TpseT1(最低)在2018年减少了13.84%。此外,我们选择的所有木霉菌株都可以促进扁豆种子的侧根生长。然而,由于叶绿素合成后期基因表达下调,新叶中叶绿素含量下降。不同木霉菌之间的拮抗作用物种导致较低的生物防治和生长促进作用,因此木霉混合物不能用作生物肥料。具有较好的生物防治和生长促进作用的TafrT52可用于日本血吸虫的生物防治。

更新日期:2020-02-22
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