Little is known about fluorescent Pseudomonas and investigations are needed to help us better understand how their species work. The aim was here to mimic what naturally occurs in environmental water containing strains isolated from mid-mountain water samples and identified as Pseudomonas fluorescens by conventional biochemical techniques. Three strains were cultured before being directly inoculated into distilled water. Surprisingly, the three cell-less extracts obtained after spinning the bacterial suspensions showed strong in vitro anti-oxidative effects against superoxide anion and hydroxyl radical but with discrepancies. The extracts obtained were found to contain antioxidant proteins among other stress proteins that were released by viable bacteria. They were identified using tandem/mass spectrometry and showed different profiles in sodium-dodecyl sulfate polyacrylamide gel electrophoresis. Bacterial identification was deepened using 16S ribonucleic acid and genome sequencing analyses to explain the differences observed between strains.

关于荧光假单胞菌知之甚少，需要进行调查以帮助我们更好地了解它们的物种如何运作。目的是模拟从中山水样中分离出并通过常规生化技术鉴定为荧光假单胞菌的环境水中天然存在的菌株。培养三株菌株，然后直接接种到蒸馏水中。令人惊讶的是，旋转细菌悬液后获得的三种无细胞提取物在体外显示出强力抗超氧阴离子和羟基自由基的氧化作用，但有差异。发现所获得的提取物除活细菌释放的其他应激蛋白外还含有抗氧化剂蛋白。使用串联/质谱法对它们进行了鉴定，并在十二烷基硫酸钠聚丙烯酰胺凝胶电泳中显示出不同的特征。使用16S核糖核酸和基因组测序分析来加深细菌鉴定，以解释菌株之间观察到的差异。