The present study was conducted to evaluate how air pre-exposure influences the responses in gills and digestive gland of Ruditapes philippinarum on subsequent Tetrabromobisphenol A (TBBPA) exposure. Firstly, clams were maintained in seawater or pre-exposed to air for 24 h, and then exposed to 0 or 100 μg/L TBBPA. Clam tissues were sampled after 1 day (T1) and 7 days (T7) of exposure to TBBPA. The results showed that in comparison with TBBPA exposure alone, air pre-exposure following TBBPA exposure reduced TBBPA accumulation in both tissues, up-regulated mRNA levels of CAT, Hsp70 and pi-GST in gills, and induced GST activity and P-glycoprotein (Pgp) mRNA level in digestive gland of clams at T1, whereas increased lipid peroxidation at T7. Overall, the findings indicate that 24-h exposure to air can activate a priming mechanism withstanding the subsequent TBBPA exposure, which is transient and may change after prolonged subsequent exposure.

进行本研究以评估空气预暴露如何影响菲律宾蛤仔g和消化腺的反应。随后的四溴双酚A（TBBPA）暴露。首先，将蛤维持在海水中或预先暴露于空气中24小时，然后暴露于0或100μg/ L的TBBPA中。在暴露于TBBPA 1天（T1）和7天（T7）之后对蛤lam组织进行采样。结果表明，与单独暴露于TBBPA相比，暴露于TBBPA之后的空气预暴露减少了两个组织中的TBBPA积累，降低了ill中CAT，Hsp70和pi-GST的mRNA水平，并诱导了GST活性和P-糖蛋白（ Pgp）在T1时蛤类消化腺中的mRNA水平，而在T7时脂质过氧化增加。总体而言，研究结果表明，暴露于空气中24小时可以抵抗随后的TBBPA暴露，这是短暂的，可能会在长时间的暴露后发生变化。