The design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 μm in width and 30 nm in height after solvent evaporation. The printed graphene oxide microelectrodes were electrochemically reduced and characterized by Raman and X-ray photoelectron spectroscopy spectroscopies in addition to microscopies. Through optimization of the electrochemical reduction parameters, differential pulse voltammetry were performed to examine the sensing of 1-naphthol (1-N), where it was revealed that reduction times had significant effects on electrode performance. The developed microelectrodes were then used as an immunoenzymatic biosensor for the detection of HT-2 mycotoxin based on carbodiimide linking of the microelectrode surface and HT-2 toxin antigen binding fragment of antibody (anti-HT2 (10) Fab). The HT-2 toxin and anti-HT2 (10) Fab reaction was reported by anti-HT2 immune complex single-chain variable fragment of antibody fused with alkaline phosphatase (anti-IC-HT2 scFv-ALP) which is able to produce an electroactive reporter – 1-N. The biosensor showed detection limits of 1.6 ng ∙ mL⁻¹ and a linear dynamic range of 6.3 – 100.0 ng ∙ mL⁻¹ within a 5 min incubation with 1-naphthyl phosphate (1-NP) substrate.

报道了喷墨印刷的电化学还原的氧化石墨烯微电极用于HT-2真菌毒素免疫酶生物传感的设计和应用。首先配制一种水性氧化石墨烯墨水，并将单滴线工作型微电极喷墨印刷到聚（2,6-萘二甲酸乙二醇酯）基底上，溶剂蒸发后的宽度为78μm，高度为30 nm。电化学还原印刷的氧化石墨烯微电极，并通过显微技术和拉曼光谱和X射线光电子能谱技术对其进行表征。通过优化电化学还原参数，执行差分脉冲伏安法检查1-萘酚（1-N）的感测，结果表明还原时间对电极性能有重大影响。然后将开发的微电极用作免疫酶生物传感器，基于微电极表面的碳二亚胺与抗体（抗HT2（10）Fab）的HT-2毒素抗原结合片段的连接来检测HT-2霉菌毒素。通过与碱性磷酸酶融合的抗体的抗HT2免疫复合物单链可变片段报道了HT-2毒素和抗HT2（10）Fab反应，该抗体能够产生电活性记者– 1-N。生物传感器的检测限为1.6 ng∙mL 通过与碱性磷酸酶融合的抗体的抗HT2免疫复合物单链可变片段报道了HT-2毒素和抗HT2（10）Fab反应，该抗体能够产生电活性记者– 1-N。生物传感器的检测限为1.6 ng∙mL 通过与碱性磷酸酶融合的抗体的抗HT2免疫复合物单链可变片段报道了HT-2毒素和抗HT2（10）Fab反应，该抗体能够产生电活性记者– 1-N。生物传感器的检测限为1.6 ng∙mL^-1和磷酸1萘酯（1-NP）底物孵育5分钟后的线性动态范围为6.3 – 100.0 ng∙mL ^-1。