BACKGROUND Canine inflammatory bowel disease (IBD) is a group of chronic gastrointestinal (GI) disorders of still largely unknown etiology. Canine IBD diagnosis is time-consuming and costly as other diseases with similar signs should be initially excluded. In human IBD microRNA (miR) expression changes have been reported in GI mucosa and blood. Thus, there is a possibility that miRs may provide insight into disease pathogenesis, diagnosis and even treatment of canine IBD. The aim of this study was to determine the colonic mucosal and serum relative expression of a miRs panel in dogs with large intestinal IBD and healthy control dogs. RESULTS Compared to healthy control dogs, dogs with large intestinal IBD showed significantly increased relative expression of miR-16, miR-21, miR-122 and miR-147 in the colonic mucosa and serum, while the relative expression of miR-185, miR-192 and miR-223 was significantly decreased. Relative expression of miR-146a was significantly increased only in the serum of dogs with large intestinal IBD. Furthermore, serum miR-192 and miR-223 relative expression correlated to disease activity and endoscopic score, respectively. CONCLUSION Our data suggest the existence of dysregulated miRs expression patterns in canine IBD and support the potential future use of serum miRs as useful noninvasive biomarkers.

中文翻译：

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犬大肠炎症性肠病结肠粘膜和血清中 microRNA 的表达。

背景技术犬炎症性肠病（IBD）是一组慢性胃肠道（GI）疾病，其病因仍很大程度上未知。犬 IBD 的诊断既耗时又昂贵，因为应首先排除具有类似症状的其他疾病。在人类 IBD 中，胃肠道粘膜和血液中的 microRNA (miR) 表达发生了变化。因此，miR 有可能为犬 IBD 的疾病发病机制、诊断甚至治疗提供见解。本研究的目的是确定患有大肠 IBD 的狗和健康对照狗的结肠粘膜和血清中 miR 组的相对表达。结果 与健康对照犬相比，大肠 IBD 犬结肠粘膜和血清中 miR-16、miR-21、miR-122 和 miR-147 的相对表达量显着升高，而 miR-185、miR-185 的相对表达量显着升高。 -192和miR-223显着下降。仅在患有大肠IBD的狗的血清中，miR-146a的相对表达显着增加。此外，血清 miR-192 和 miR-223 相对表达分别与疾病活动度和内镜评分相关。结论 我们的数据表明犬 IBD 中存在失调的 miR 表达模式，并支持未来可能使用血清 miR 作为有用的非侵入性生物标志物。