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Enhanced bioactive compound production in broccoli cells due to coronatine and methyl jasmonate is linked to antioxidative metabolism
Journal of Plant Physiology ( IF 4.0 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.jplph.2020.153136
Pedro Joaquín Sánchez-Pujante 1 , Matteo Gionfriddo 2 , Ana Belén Sabater-Jara 1 , Lorena Almagro 1 , María Angeles Pedreño 1 , Pedro Diaz-Vivancos 1
Affiliation  

Elicited broccoli suspension-cultured cells (SCC) provide a useful system for obtaining bioactive compounds, including glucosinolates (GS) and phenolic compounds (PCs). In this work, coronatine (Cor) and methyl jasmonate (MJ) were used to increase the bioactive compound production in broccoli SCC. Although the use of Cor and MJ in secondary metabolite production has already been described, information concerning how elicitors affect cell metabolism is scarce. It has been suggested that Cor and MJ trigger defence reactions affecting the antioxidative metabolism. In the current study, the concentration of 0.5 μM Cor was the most effective treatment for increasing both the total antioxidant capacity (measured as ferulic acid equivalents) and glucosinolate content in broccoli SCC. The elicited broccoli SCC also showed higher polyphenol oxidase activity than the control cells. Elicitation altered the antioxidative metabolism of broccoli SCC, which displayed biochemical changes in antioxidant enzymes, a decrease in the glutathione redox state and an increase in lipid peroxidation levels. Furthermore, we studied the effect of elicitation on the protein profile and observed an induction of defence-related proteins. All of these findings suggest that elicitation not only increases bioactive compound production, but it also leads to mild oxidative stress in broccoli SCC that could be an important factor triggering the production of these compounds.

中文翻译:

由于冠状素和茉莉酸甲酯,西兰花细胞中生物活性化合物的产生增强与抗氧化代谢有关

诱导的西兰花悬浮培养细胞 (SCC) 为获得生物活性化合物提供了一个有用的系统,包括硫代葡萄糖苷 (GS) 和酚类化合物 (PC)。在这项工作中,冠花碱 (Cor) 和茉莉酸甲酯 (MJ) 用于增加西兰花 SCC 中生物活性化合物的产生。尽管已经描述了 Cor 和 MJ 在次生代谢产物生产中的使用,但关于激发子如何影响细胞代谢的信息很少。已经提出 Cor 和 MJ 触发影响抗氧化代谢的防御反应。在目前的研究中,0.5 μM C​​or 的浓度是提高西兰花 SCC 中总抗氧化能力(以阿魏酸当量衡量)和硫代葡萄糖苷含量的最有效处理方法。引发的西兰花 SCC 也显示出比对照细胞更高的多酚氧化酶活性。诱导改变了西兰花 SCC 的抗氧化代谢,显示出抗氧化酶的生化变化、谷胱甘肽氧化还原状态的降低和脂质过氧化水平的增加。此外,我们研究了诱导对蛋白质谱的影响,并观察到防御相关蛋白质的诱导。所有这些发现表明,诱导不仅会增加生物活性化合物的产生,而且还会导致西兰花 SCC 中的轻度氧化应激,这可能是触发这些化合物产生的重要因素。谷胱甘肽氧化还原状态的降低和脂质过氧化水平的增加。此外,我们研究了诱导对蛋白质谱的影响,并观察到防御相关蛋白质的诱导。所有这些发现表明,诱导不仅会增加生物活性化合物的产生,而且还会导致西兰花 SCC 中的轻度氧化应激,这可能是触发这些化合物产生的重要因素。谷胱甘肽氧化还原状态的降低和脂质过氧化水平的增加。此外,我们研究了诱导对蛋白质谱的影响,并观察到防御相关蛋白质的诱导。所有这些发现表明,诱导不仅会增加生物活性化合物的产生,而且还会导致西兰花 SCC 中的轻度氧化应激,这可能是触发这些化合物产生的重要因素。
更新日期:2020-05-01
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