Promoting thermogenesis of brown and beige adipocytes increases energy expenditure, which is a critical approach to prevent obesity. In the present study, we elucidated the effect of the citrus polymethoxyflavones on adaptive thermogenesis in high fat diet induced obesity mouse model. The PMFs from Citrus aurantium L. analyzed by UPLC are mainly nobiletin, tangeretin and sinensetin. Dietary supplementation of 0.2% citrus PMFs with high fat diet (w/w) for 12 weeks markedly reduced body weight gain, white adipose weight and size. The cold tolerance test showed that PMFs supplementation maintained body temperature and elevated nonshivering thermogenesis. The Q-PCR and IHC revealed that PMFs promote expression of thermogenesis genes and mitochondrial biogenesis of BAT and iWAT. Mechanistically, we found that PMFs activated AMPK/PGC1α signaling axis. This study demonstrated that citrus PMFs could prevent obesity by activating thermogenesis of BAT and iWAT. PMFs may use as a novel dietary supplemental approach for obesity.

促进棕色和米色脂肪细胞的生热作用会增加能量消耗，这是预防肥胖的关键方法。在本研究中，我们阐明了柑橘类聚甲氧基黄酮在高脂饮食诱导的肥胖小鼠模型中对适应性生热的作用。柑桔的PMF通过UPLC分析的L.主要是诺比列汀，橘皮苷和芥子素。饮食中添加0.2％柑橘PMF和高脂饮食（w / w）持续12周，可显着降低体重增加，白色脂肪的重量和大小。耐寒性试验表明，补充PMF可以保持体温，并增加非颤抖的生热作用。Q-PCR和IHC显示PMF促进BAT和iWAT的生热基因表达和线粒体生物发生。从机理上讲，我们发现PMF激活了AMPK /PGC1α信号轴。这项研究表明，柑橘类PMF可以通过激活BAT和iWAT的生热作用来预防肥胖。PMF可以用作肥胖症的新型饮食补充方法。