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Fluorogen activating protein toolset for protein trafficking measurements.
Traffic ( IF 3.6 ) Pub Date : 2020-02-20 , DOI: 10.1111/tra.12722
Lydia A Perkins 1 , Marcel P Bruchez 2, 3, 4
Affiliation  

Throughout the past decade the use of fluorogen activating proteins (FAPs) has expanded with several unique reporter dyes that support a variety of methods to specifically quantify protein trafficking events. The platform's capabilities have been demonstrated in several systems and shared for widespread use. This review will highlight the current FAP labeling techniques for protein traffic measurements and focus on the use of the different designed fluorogenic dyes for selective and specific labeling applications.

中文翻译:


用于蛋白质运输测量的荧光激活蛋白质工具集。



在过去的十年中,荧光激活蛋白 (FAP) 的使用不断扩大,多种独特的报告染料支持多种方法来特异性量化蛋白质运输事件。该平台的功能已在多个系统中得到展示并共享以供广泛使用。本综述将重点介绍当前用于蛋白质流量测量的 FAP 标记技术,并重点关注不同设计的荧光染料在选择性和特定标记应用中的使用。
更新日期:2020-03-12
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