Chromosomes and chromosomal gene delivery vectors, human/mouse artificial chromosomes (HACs/MACs), can introduce megabase-order DNA sequences into target cells and are used for applications including gene mapping, gene expression control, gene/cell therapy, and the development of humanized animals and animal models of human disease. Microcell-mediated chromosome transfer (MMCT), which enables chromosome transfer from donor cells to target cells, is a key technology for these applications. In this review, we summarize the principles of gene transfer with HACs/MACs; their engineering, characteristics, and utility; and recent advances in the chromosome transfer technology.

染色体和染色体基因传递载体，人类/小鼠人工染色体（HACs / MACs），可以将巨碱基顺序的DNA序列引入靶细胞，并用于包括基因作图，基因表达控制，基因/细胞治疗以及肝癌的开发等应用。人性化动物和人类疾病的动物模型。微细胞介导的染色体转移（MMCT）是使染色体从供体细胞转移到靶细胞的关键技术，是这些应用的关键技术。在这篇综述中，我们总结了利用HACs / MACs进行基因转移的原理。其工程，特性和效用；和染色体转移技术的最新进展。