Human umbilical cord mesenchymal stem cell-derived and dermal fibroblast-derived extracellular vesicles protect dermal fibroblasts from ultraviolet radiation-induced photoaging in vitro.,Photochemical & Photobiological Sciences

当前位置： X-MOL 学术 › Photochem. Photobiol. Sci. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Human umbilical cord mesenchymal stem cell-derived and dermal fibroblast-derived extracellular vesicles protect dermal fibroblasts from ultraviolet radiation-induced photoaging in vitro.
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2020-03-03 , DOI: 10.1039/c9pp00421a
Mingwu Deng ₁ , T Ziyou Yu ₁ , Dong Li ₁ , Xiangsheng Wang ₁ , Guangdong Zhou ₁ , Wei Liu ₁ , Yilin Cao ₁ , Wanyao Xia ₁ , Wei Li ₁ , Wen Jie Zhang ₁

Affiliation

Ultraviolet B (UVB) radiation is a major cause of aging in dermal fibroblasts. Human umbilical cord mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) show antioxidant activity. In this study, the anti-aging effects of MSC-EVs on dermal fibroblast photoaging induced by UVB radiation were evaluated, and the effects of extracellular vesicles derived from dermal fibroblasts (Fb-EVs) were compared. Human umbilical cord mesenchymal stem cells and human dermal fibroblasts were cultured, and MSC-EVs and Fb-EVs were isolated and characterized. Human dermal fibroblasts were cultured in the absence or presence of different concentrations of EVs 24 hours prior to UVB radiation exposure. Cell proliferation and cell cycle were evaluated, and senescent cells and intracellular ROS were detected. The expressions of matrix metalloproteinase-1 (MMP-1), extracellular matrix protein collagen type 1 (Col-1), and antioxidant proteins such as glutathione peroxidase 1 (GPX-1), superoxide dismutase (SOD), and catalase were also analyzed. Pretreatment with MSC-EVs or Fb-EVs significantly inhibited the production of ROS induced by UVB radiation, increased dermal fibroblast proliferation, protected cells against UVB-induced cell death and cell cycle arrest, and remarkably decreased the percentage of aged cells. Pretreatment with MSC-EVs or Fb-EVs promoted the expressions of GPX-1 and Col-1 and decreased the expression of MMP-1. Both MSC-EVs and Fb-EVs protected dermal fibroblasts from UVB-induced photoaging, likely through their antioxidant activity.

中文翻译：

人脐带间充质干细胞和真皮成纤维细胞外囊泡可保护真皮成纤维细胞免受紫外线辐射引起的体外光老化。

紫外线B（UVB）辐射是皮肤成纤维细胞衰老的主要原因。人脐带间充质干细胞来源的细胞外囊泡（MSC-EVs）显示抗氧化活性。在这项研究中，评估了MSC-EVs对UVB辐射诱导的皮肤成纤维细胞光老化的抗衰老作用，并比较了源自真皮成纤维细胞（Fb-EVs）的细胞外囊泡的作用。培养人脐带间充质干细胞和人真皮成纤维细胞，并分离和鉴定MSC-EV和Fb-EV。在暴露于UVB之前，在不存在或存在不同浓度的EV的情况下培养人皮肤成纤维细胞24小时。评价细胞增殖和细胞周期，并检测衰老细胞和细胞内ROS。基质金属蛋白酶-1（MMP-1）的表达，还分析了细胞外基质蛋白1型胶原蛋白（Col-1）和抗氧化剂蛋白，如谷胱甘肽过氧化物酶1（GPX-1），超氧化物歧化酶（SOD）和过氧化氢酶。用MSC-EV或Fb-EV进行预处理可显着抑制UVB辐射诱导的ROS的产生，增加皮肤成纤维细胞增殖，保护细胞免受UVB诱导的细胞死亡和细胞周期阻滞，并显着降低衰老细胞的百分比。MSC-EVs或Fb-EVs预处理可促进GPX-1和Col-1的表达，并降低MMP-1的表达。MSC-EV和Fb-EV都可能通过其抗氧化活性来保护皮肤成纤维细胞免受UVB诱导的光老化。和过氧化氢酶也进行了分析。用MSC-EV或Fb-EV进行预处理可显着抑制UVB辐射诱导的ROS的产生，增加皮肤成纤维细胞增殖，保护细胞免受UVB诱导的细胞死亡和细胞周期阻滞，并显着降低衰老细胞的百分比。MSC-EVs或Fb-EVs预处理可促进GPX-1和Col-1的表达，并降低MMP-1的表达。MSC-EV和Fb-EV都可能通过其抗氧化活性来保护皮肤成纤维细胞免受UVB诱导的光老化。和过氧化氢酶也进行了分析。用MSC-EV或Fb-EV进行预处理可显着抑制UVB辐射诱导的ROS的产生，增加皮肤成纤维细胞增殖，保护细胞免受UVB诱导的细胞死亡和细胞周期阻滞，并显着降低衰老细胞的百分比。MSC-EVs或Fb-EVs预处理可促进GPX-1和Col-1的表达，并降低MMP-1的表达。MSC-EV和Fb-EV都可能通过其抗氧化活性来保护皮肤成纤维细胞免受UVB诱导的光老化。MSC-EVs或Fb-EVs预处理可促进GPX-1和Col-1的表达，并降低MMP-1的表达。MSC-EV和Fb-EV都可能通过其抗氧化活性来保护皮肤成纤维细胞免受UVB诱导的光老化。MSC-EVs或Fb-EVs预处理可促进GPX-1和Col-1的表达，并降低MMP-1的表达。MSC-EV和Fb-EV都可能通过其抗氧化活性来保护皮肤成纤维细胞免受UVB诱导的光老化。

更新日期：2020-03-19

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11