Abstract

Colorectal cancer (CRC) is still a major killer, mainly due to the lack of early detection biomarker with sensitivity/specificity. Conventional histopathology is the gold standard approach in practice, but there are still some issues (false diagnosis and long waiting period). Aggregation-induced emission (AIE) is a novel photophysical phenomenon which has been widely utilized for biological imaging in vivo, including malignant cells, showing remarkable imaging contrast and enhanced emission in high concentrated state. However, it is unclear if AIE dyes can identify malignant cells specifically in vivo. A great challenge for specific labeling of malignant cells is probably ascribed to the complex compositions in tumor tissues. Herein, an AIE dye-based mitochondria-targeted histological staining strategy was employed to localize cancer and the metastatic track in the fresh colorectal cancer. This novel approach holds great potential to revolutionize the clinical diagnosis and intervention for devastating malignancy.

中文翻译：

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聚集诱导的发光原用于体内恶性肿瘤的特异性鉴定

摘要

结直肠癌（CRC）仍然是主要杀手，主要是由于缺乏具有敏感性/特异性的早期检测生物标志物。传统的组织病理学是实践中的金标准方法，但是仍然存在一些问题（错误的诊断和漫长的等待时间）。聚集诱导发射（AIE）是一种新颖的光物理现象，已广泛用于体内生物成像，包括恶性细胞，在高浓度状态下显示出显着的成像对比度并增强了发射。但是，尚不清楚AIE染料是否可以在体内特异性鉴定恶性细胞。特异性标记恶性细胞的巨大挑战可能归因于肿瘤组织中的复杂成分。在本文中，基于AIE染料的线粒体靶向组织学染色策略被用来定位癌症和新鲜结直肠癌的转移轨迹。这种新颖的方法具有巨大的潜力，可以彻底改变破坏性恶性肿瘤的临床诊断和干预措施。