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Comparative genetic and physiological characterisation of Pectinatus species reveals shared tolerance to beer-associated stressors but halotolerance specific to pickle-associated strains.
Food Microbiology ( IF 4.5 ) Pub Date : 2020-02-21 , DOI: 10.1016/j.fm.2020.103462
Timo Kramer 1 , Philip Kelleher 2 , Julia van der Meer 3 , Tadhg O'Sullivan 4 , Jan-Maarten A Geertman 4 , Sylvia H Duncan 1 , Harry J Flint 1 , Petra Louis 1
Affiliation  

Obligate anaerobic bacteria from the genus Pectinatus have been known to cause beer spoilage for over 40 years. Whole genome sequencing was performed on eleven beer spoilage strains (nine Pectinatus frisingensis, one Pectinatus cerevisiiphilus and one Pectinatus haikarae isolate), as well as two pickle spoilage species (Pectinatus brassicae MB591 and Pectinatus sottacetonis MB620) and the tolerance of all species to a range of environmental conditions was tested. Exploration of metabolic pathways for carbohydrates, amino acids and vitamins showed little difference between beer spoilage- and pickle spoilage-associated strains. However, genes for certain carbohydrate- and sulphur-containing amino acid-associated enzymes were only present in the beer spoilage group and genes for specific transporters and regulatory genes were uniquely found in the pickle spoilage group. Transporters for compatible solutes, only present in pickle-associated strains, likely explain their experimentally observed higher halotolerance compared to the beer spoilers. Genes involved in biofilm formation and ATP Binding Cassette (ABC) transporters potentially capable of exporting hop-derived antimicrobial compounds were found in all strains. All species grew in the presence of alcohol up to 5% alcohol by volume (ABV) and hops extract up to 80 ppm of iso-α-acids. Therefore, the species isolated from pickle processes may pose novel hazards in brewing.

中文翻译:

比较的遗传学和生理学表征的果蝇物种揭示了对啤酒相关应激源的共有耐受性,但对与酱菜相关菌株特有的耐盐性。

已知来自Pectinatus属的专性厌氧细菌会导致啤酒变质40多年。全基因组测序对11种啤酒腐败菌株(9种啤酒菌,1种酿酒酵母和1个海藻分离菌)以及2种酱菜腐败菌(芸苔菌MB591和sottacetonis MB620)进行了全基因组测序,并且所有物种对一定范围的耐受性测试了环境条件。对碳水化合物,氨基酸和维生素的代谢途径的探索显示,啤酒腐败变质和腌菜腐败变质相关菌株之间的差异很小。然而,某些与碳水化合物和含硫氨基酸相关的酶的基因仅存在于啤酒腐败组中,而特定转运蛋白和调节基因的基因在腌制腐败组中独有。仅在与酱菜相关的菌株中存在的相容性溶质转运蛋白,可能解释了他们实验观察到的比啤酒扰流剂更高的耐卤性。在所有菌株中均发现了与生物膜形成和ATP结合盒(ABC)转运蛋白有关的基因,这些基因可能能够输出啤酒花衍生的抗菌化合物。所有物种都在酒精存在下生长,酒精含量最高可达5%体积酒精(ABV),而啤酒花可提取高达80 ppm的异α-酸。因此,从腌制过程中分离出来的物种可能在酿造中带来新的危害。仅存在于泡菜相关菌株中的细菌,可能解释了它们在实验中观察到的比啤酒扰流剂更高的耐卤性。在所有菌株中均发现了与生物膜形成和ATP结合盒(ABC)转运蛋白有关的基因,这些基因可能能够输出啤酒花衍生的抗菌化合物。所有物种都在酒精存在下生长,酒精含量最高可达5%体积酒精(ABV),而啤酒花可提取高达80 ppm的异α-酸。因此,从腌制过程中分离出来的物种可能在酿造中带来新的危害。仅存在于泡菜相关菌株中的细菌,可能解释了它们在实验中观察到的比啤酒扰流剂更高的耐卤性。在所有菌株中均发现了与生物膜形成和ATP结合盒(ABC)转运蛋白有关的基因,这些基因可能能够输出啤酒花衍生的抗菌化合物。所有物种都在酒精存在下生长,酒精含量最高可达5%体积酒精(ABV),而啤酒花可提取高达80 ppm的异α-酸。因此,从腌制过程中分离出来的物种可能在酿造中带来新的危害。所有物种都在酒精存在下生长,酒精含量最高可达5%体积酒精(ABV),而啤酒花可提取高达80 ppm的异α-酸。因此,从腌制过程中分离出来的物种可能在酿造中带来新的危害。所有物种都在酒精存在下生长,酒精含量最高可达5%(体积)(ABV),啤酒花可萃取出80 ppm的异α-酸。因此,从腌制过程中分离出来的物种可能在酿造中带来新的危害。
更新日期:2020-02-21
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