INTRODUCTION MicroRNAs have a significant role in the pathogenesis of preeclampsia. Circulating microRNAs could represent a potential biomarker for preeclampsia. The aim of this study was to evaluate plasma miR210-3p and miR518b in preeclampsia and healthy pregnancy for the first time by digital droplet PCR (ddPCR). METHODS Thirty-six pregnant women (seventeen healthy pregnancies, nineteen preeclampsia patients) were involved from the Clinic for Gynaecology and Obstetrics "Narodni front" in Belgrade, Serbia. Plasma miR210-3p, miR518b and cel-miR-39 as a spike-in control were measured by ddPCR. RESULTS MiR518b was significantly elevated in preeclampsia compared to a healthy pregnancy (P = 0.034; 0.302(0.217-0.421) vs. 0.171(0.110-0.266)). MiR210-3p showed no significant difference between the two groups (P = 0.951). The adjustment of miR518b was made for a gestational age and smoking status and the difference between the preeclampsia and healthy pregnancy group was more significant (P = 0.026; 0.300(0.216-0.419) vs. 0.172(0.121-0.245)). Plasma miR-518b was significantly higher in the group of preeclampsia patients with proteinuria above the 75th percentile for the group (P = 0.033), in women who smoked (P = 0.039), and was positively related to uric acid in preeclampsia (P = 0.018, r = 0.536). Plasma miR518b was able to significantly discriminate between preeclampsia and healthy pregnancy, yielding AUC of 0.712 (95%CI:0.539-0.891), P = 0.028. CONCLUSIONS In this study plasma microRNA were measured for the first time in preeclampsia and healthy pregnancies with ddPCR. Placenta-specific miR-518b could serve as a potential biomarker for discriminating preeclampsia and healthy pregnancy, which should be confirmed on a larger study population. This study has failed to confirm the same potential for miR210-3p.

中文翻译：

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胎盘特异性血浆miR518b是先兆子痫的潜在生物标志物。

引言MicroRNA在先兆子痫的发病机理中具有重要作用。循环microRNAs可能代表先兆子痫的潜在生物标志物。这项研究的目的是通过数字液滴PCR（ddPCR）首次评估先兆子痫和健康妊娠中的血浆miR210-3p和miR518b。方法来自塞尔维亚贝尔格莱德的妇产科诊所“ Narodni front”的三十六名孕妇（十七名健康怀孕，十九名先兆子痫患者）被纳入研究。通过ddPCR测量血浆miR210-3p，miR518b和cel-miR-39作为加标对照。结果与健康妊娠相比，先兆子痫中的MiR518b显着升高（P = 0.034; 0.302（0.217-0.421）vs. 0.171（0.110-0.266））。MiR210-3p在两组之间没有显着差异（P = 0.951）。根据胎龄和吸烟状况对miR518b进行了调整，子痫前期和健康妊娠组之间的差异更为显着（P = 0.026； 0.300（0.216-0.419）； 0.172（0.121-0.245））。子痫前期组蛋白尿高于该组第75个百分位数的患者（P = 0.033），吸烟女性（P = 0.039）的血浆miR-518b显着升高，并且与子痫前期的尿酸呈正相关（P = 0.018，r = 0.536）。血浆miR518b能够明显区分先兆子痫和健康妊娠，产生的AUC为0.712（95％CI：0.539-0.891），P = 0.028。结论在这项研究中，首次通过子痫性先兆子痫和健康孕妇测量了血浆microRNA。胎盘特异性miR-518b可以作为区分先兆子痫和健康妊娠的潜在生物标志物，应在更大的研究人群中加以证实。这项研究未能证实miR210-3p具有相同的潜力。