In 2018, more than 50 cases of horse death by equine atypical myopathy (AM) were reported in the Czech Republic. This disease is often associated with the toxin hypoglycine A (HGA), which is found in several maple plant materials. To monitor this toxin in products of these trees that grow in or around horse pastures, a rapid and inexpensive analytical method that can provide the required accuracy is needed.

Until now, maple samples have been prepared for gas chromatography using time-consuming methods, with preparation processes taking longer than 1 h. In this work, a shorter method (25 min) with an accuracy of 90–94 %, reproducibility of 2–5%, precision of 3–9%, and linearity, with an R² of 0.999, is presented. This sample preparation consists of a procedure without an SPE extraction step and consumes a lower volume of solvent during the extraction. The limit of quantitation for HGA in plant material was improved from 0.5 μg/g of plant material in previous studies to 0.2 μg/g. The method was validated according to the guideline CD 2002/657/EC and ISO 17025, and was found to have good performance characteristics.

This simple and rapid method was tested for the monitoring of hypoglycine A level in maple sycamore plant material (seeds, seedlings, and leaves) during the entire growth of the trees.

2018年，捷克共和国报告了超过50例马非典型马病（AM）导致的马死亡案例。这种疾病通常与几种枫树植物材料中的毒素次甘氨酸A（HGA）有关。为了监测在马牧场内或周围生长的这些树木的产品中的这种毒素，需要一种能够提供所需准确性的快速且廉价的分析方法。

到目前为止，已经使用费时的方法制备了用于气相色谱的枫木样品，制备过程耗时超过1小时。在这项工作中，提出了一种较短的方法（25分钟），其准确度为90–94％，重现性为2–5％，精度为3–9％和线性，R ²为0.999。该样品制备过程不包括SPE萃取步骤，并且在萃取过程中消耗的溶剂量较少。植物材料中HGA的定量限从先前研究中的0.5μg/ g植物材料提高到0.2μg/ g。该方法已根据准则CD 2002/657 / EC和ISO 17025进行了验证，并被发现具有良好的性能特征。

测试了此简单而快速的方法，以监测树木整个生长过程中枫树无花果植物材料（种子，幼苗和叶子）中的次要甘氨酸A水平。