Abstract The composite preparation combining carbon nanotubes, biological systems and polymers to form homogenous and stable assemblies is an innovative research area with great significant for practical application of carbon nanotubes in printing, packaging and sensors areas. In this work, multi-walled carbon nanotubes (MWCNTs) was dispersed in lysozyme solution via sonification process, and then mixed with waterborne polyurethane (WPU) through a facile process. The non-covalent interaction among lysozyme, MWCNTs and WPU chains was investigated using the methods of scanning electron microscopy, transmission electron microscopy, UV–vis spectroscopy, Raman spectroscopy, fluorescence anisotropy, and X-ray diffraction. It suggests that lysozyme may adsorb nanotubes to form a certain orientation with the function of charge-π interaction, and there may be 2∼5 lysozyme biomolecules bound to hemi-CNTs side. Moreover, the homogeneous, stable and spherical nanoparticles of WPU are significantly stimulative for the stability of the MWCNTs/lysozyme/WPU composite through non-covalent interactions.

中文翻译：

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溶菌酶@碳纳米管/水性聚氨酯复合材料的制备、表征及其在印刷油墨中的潜在应用

摘要 将碳纳米管、生物系统和聚合物结合形成均质稳定的组装体的复合制备是一个创新的研究领域，对于碳纳米管在印刷、包装和传感器领域的实际应用具有重要意义。在这项工作中，多壁碳纳米管（MWCNTs）通过超声处理分散在溶菌酶溶液中，然后通过一个简单的过程与水性聚氨酯（WPU）混合。使用扫描电子显微镜、透射电子显微镜、紫外-可见光谱、拉曼光谱、荧光各向异性和 X 射线衍射的方法研究了溶菌酶、多壁碳纳米管和 WPU 链之间的非共价相互作用。这表明溶菌酶可以通过电荷-π相互作用的作用吸附纳米管形成一定的取向，并且可能有2~5个溶菌酶生物分子结合到半碳纳米管一侧。此外，WPU 的均质、稳定和球形纳米粒子通过非共价相互作用显着促进 MWCNTs/溶菌酶/WPU 复合材料的稳定性。