The present study aimed to explore the biosynthesis and characterization of silver nanoparticles (AgNPs) from Moringa oleifera flower (MOF) extract and its antimicrobial and sensing properties. The prepared AgNPs were characterized by Transmission Electron Microscopy (TEM), UV–visible spectral analysis (UV–vis), X-Ray Diffraction (X-RD), Energy Dispersive Spectroscopy (EDS), and Fourier Infra-Red Spectroscopy (FTIR) respectively and antimicrobial properties were determined by dis diffusion techniques. Face-Centered Cubic (FCC) lattice of the AgNPs was observed in X-RD pattern. FTIR measurement evidenced the band pattern at 686, 1653, 2062 and 3456 cm⁻¹ proved the presence of proteins and phenolic components in MOF responsible for reduction. TEM analysis indicated the formation of monodispersed spherical particles with 8 nm. UV–vis of the prepared AgNPs authenticated the surface plasmon resonance (SPR) at 429 nm and stable for six months. AgNPs have produced highest zone of inhibition (ZOI) of 17 mm and 29 mm against Klebsiella pneumoniae and Staphylococcus aureus respectively. In addition, the AgNPs effectively detected the presence of Copper ions from 1 mM to 12 mM concentrations.in Copper sensitivity of these biosynthesized nanoparticles was carried out by optical sensor based SPR. Thus the obtained antimicrobial and optical properties, suggested the use of obtained AgNPs in water purification.

本研究旨在探讨辣木花（MOF）提取物中银纳米颗粒（AgNPs）的生物合成和表征及其抗菌和传感特性。通过透射电子显微镜（TEM），紫外可见光谱分析（UV-vis），X射线衍射（X-RD），能量色散光谱（EDS）和傅里叶红外光谱（FTIR）对制备的AgNP进行表征分别通过分散技术确定抗菌性能。AgNPs的面心立方（FCC）晶格在X-RD模式中观察到。FTIR测量证明在686、1653、2062和3456 cm ^-1处的能带模式证明了MOF中存在蛋白质和酚类成分负责减少。TEM分析表明形成了具有8nm的单分散球形颗粒。制备的AgNP的紫外可见光可在429 nm处验证表面等离振子共振（SPR），并稳定六个月。AgNPs对肺炎克雷伯菌和金黄色葡萄球菌分别具有最高的抑制区（ZOI），分别为17 mm和29 mm 。此外，AgNPs有效地检测到了1 mM至12 mM浓度的铜离子。在这些生物合成的纳米颗粒的铜敏感性是通过基于光学传感器的SPR进行的。因此，所获得的抗微生物和光学性质表明所获得的AgNP在水净化中的用途。