Abstract Zygosaccharomyces rouxii and Tetragenococcus halophilus exhibit remarkable salt tolerance and play roles in high-salt fermented food production. This study investigated the effect of co-culture with T. halophilus on Z. rouxii based on analysis of the viability of Z. rouxii in high-salt environments, the plasma membrane integrity, Na+, K+-ATPase activity, amino acid content of Z. rouxii cell after salt stress and organic acids assay. The results showed both T. halophilus broth supernatant and intracellular component of T. halophilus increased the viability of Z. rouxii in the 12 % environment. Co-cultured Z. rouxii cells maintained better plasma membrane integrity and lowered Na+, K+-ATPase activity than single-cultured after salt stress. Co-cultured Z. rouxii cells exhibited higher contents of aspartic acid, threonine, serine, asparagine, glutamic acid, alanine, α-amino-n-butyric acid, methionine, homo-cystine, arginine and proline compared with single-cultured after salt stress. More contents of propionic acid, lactic acid and L-pyroglutamic acid and lower contents of L-malic acid and citric acid were detected in co-culture broth. This study shows preculture of T. halophilus and then co-culture with Z. rouxii enhanced the viability of Z. rouxii in high-salt environment. The results may contribute to further understand the interactions between Z. rouxii and T. halophilus in high-salt environments.

中文翻译：

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与嗜盐四联球菌共培养改变了鲁氏接合酵母对盐胁迫的反应

摘要 Zygosaccharomyces rouxii 和Tetragenococcus halophilus 表现出显着的耐盐性，在高盐发酵食品生产中发挥作用。本研究通过分析红曲霉在高盐环境中的生存力、质膜完整性、Na+、K+-ATPase 活性、Z. 的氨基酸含量，研究了与 T. halophilus 共培养对 Z. rouxii 的影响。 . 盐胁迫和有机酸测定后的rouxii细胞。结果显示嗜盐嗜盐菌肉汤上清液和嗜盐嗜盐菌的细胞内成分均提高了 12% 环境中的 Z.rouxii 的生存力。与盐胁迫后的单一培养相比，共培养的鲁氏酵母细胞保持了更好的质膜完整性并降低了 Na+、K+-ATPase 活性。共培养的 Z. rouxii 细胞显示出更高含量的天冬氨酸、苏氨酸、丝氨酸、天冬酰胺、与盐胁迫后单独培养相比，谷氨酸、丙氨酸、α-氨基-正丁酸、蛋氨酸、高胱氨酸、精氨酸和脯氨酸。共培养肉汤中丙酸、乳酸和L-焦谷氨酸含量较高，L-苹果酸和柠檬酸含量较低。本研究表明，先培养 T. halophilus，然后与 Z. rouxii 共培养提高了 Z. rouxii 在高盐环境中的生存能力。该结果可能有助于进一步了解高盐环境中 Z. rouxii 和 T. halophilus 之间的相互作用。在共培养肉汤中检测到乳酸和L-焦谷氨酸，而L-苹果酸和柠檬酸的含量较低。本研究表明，先培养 T. halophilus 然后与 Z. rouxii 共培养提高了 Z. rouxii 在高盐环境中的生存能力。该结果可能有助于进一步了解高盐环境中 Z. rouxii 和 T. halophilus 之间的相互作用。在共培养肉汤中检测到乳酸和L-焦谷氨酸，而L-苹果酸和柠檬酸的含量较低。本研究表明，先培养 T. halophilus，然后与 Z. rouxii 共培养提高了 Z. rouxii 在高盐环境中的生存能力。该结果可能有助于进一步了解高盐环境中 Z. rouxii 和 T. halophilus 之间的相互作用。