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Improved lignocellulolytic enzyme production and antioxidant extraction using solid‐state fermentation of olive pomace mixed with winery waste
Biofuels, Bioproducts and Biorefining ( IF 3.2 ) Pub Date : 2019-12-23 , DOI: 10.1002/bbb.2073
Diogo Filipe 1 , Helena Fernandes 1 , Carolina Castro 1 , Helena Peres 1 , Aires Oliva‐Teles 1 , Isabel Belo 2 , José M. Salgado 2
Affiliation  

Olive pomace is characterized by its low nutritional value and high phenolic content, which hinders its direct use as animal feed, fertilizer, or as a substrate in bioprocesses such as solid‐state fermentation (SSF). A possible strategy for bioprocessing olive pomace by SSF is the mixture of olive mill wastes with other wastes produced in the same region, such as winery wastes. This may improve the production of bioactive compounds like enzymes and antioxidant phenolics. A simplex‐centroid design was used to evaluate the use of olive mill and winery wastes alone or in combination as a substrate for SSF with Aspergillus niger and Aspergillus ibericus. Synergistic effects of combinations of crude olive pomace (COP), exhausted olive pomace (EOP), vine trimming shoots (VTS), and exhausted grape marc (EGM) were observed in the production of xylanases, cellulases, β‐glucosidases, and in the variation in total phenolics and antioxidant activity of SFF extracts. A multiple response optimization was carried out, leading to the following optimal mixture of substrates: for A. niger, 23% (w/w) COP, 30% EGM, 33% VTS, 14% EOP; for A. ibericus, 30% EGM, 36% VTS, 34% EOP. The scale‐up to tray bioreactor with optimal substrate made it possible to achieve the maximum xylanase, cellulase, and β‐glucosidase production of 189.1 ± 26.7, 56.3 ± 2.1 and 10.9 ± 0.8 U/g, respectively. The antioxidant activity of fermented wastes was also improved 2.2‐fold as compared with unfermented wastes. Thus, a combination of olive mill and winery wastes in SSF is a potential strategy to increase their value and to develop a circular strategy in these industries. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd.

中文翻译:

橄榄果渣与酿酒厂废料的固态发酵可改善木质纤维素分解酶的生产和抗氧化剂的提取

橄榄果渣的特点是营养价值低,酚含量高,这阻碍了它直接用作动物饲料,肥料或生物发酵(SSF)等生物过程的底物。SSF对橄榄果渣进行生物加工的一种可行策略是将橄榄厂废料与同一地区生产的其他废料(如酒厂废料)混合。这可以提高生物活性化合物(如酶和抗氧化剂酚)的生产。单形质心设计用于评估橄榄研磨厂和酿酒厂废物单独使用或与黑曲霉艾伯曲霉联合用作SSF基质的用途。在木聚糖酶,纤维素酶,β-葡萄糖苷酶的生产以及在SFF提取物的总酚和抗氧化活性的变化。进行了多重响应优化,得到了以下最佳的底物混合物:对于黑曲霉,23%(w / w)COP,30%EGM,33%VTS,14%EOP;对于A. ibericus而言,EGM为30%,VTS为36%,EOP为34%。放大到具有最佳底物的塔盘生物反应器可以实现最大的木聚糖酶,纤维素酶和β葡糖苷酶的产量分别为189.1±26.7、56.3±2.1和10.9±0.8 U / g。与未发酵废物相比,发酵废物的抗氧化活性也提高了2.2倍。因此,在SSF中将橄榄厂和酿酒厂废物组合在一起是增加其价值并制定这些行业循环战略的潜在策略。©2019年化学工业协会和John Wiley&Sons,Ltd.
更新日期:2020-02-21
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