A novel signal‐on fluorescent aptasensor for ochratoxin A detection based on RecJf exonuclease‐induced signal amplification,Journal of the Chinese Chemical Society

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A novel signal‐on fluorescent aptasensor for ochratoxin A detection based on RecJf exonuclease‐induced signal amplification
Journal of the Chinese Chemical Society ( IF 1.6 ) Pub Date : 2020-02-20 , DOI: 10.1002/jccs.201900423
Min Wei ₁ , Xing He ₁ , Yanli Xie ₁

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This article describes a simple and homogeneous fluorescent aptasensor for the detection of ochratoxin A (OTA). With its high specificity and simplicity; RecJ_f exonuclease is used to cleave DNA strand of the FAM‐aptamer/OTA complex and realize target recycling signal amplification. In order to avoid the loss of reaction system, magnetic beads (MBs) are added only once at the last experimental step. This proposed fluorescent aptasensor showed the higher sensitivity in the range of 0.1–100 ng/mL with LOD of 0.056 ng/mL, and the good selectivity against other interfering toxins. The feasibility of the prepared aptasensor was studied by detecting OTA in spiked liquor and cereal samples. The obtained average recoveries ranged from 92% to 115%. This study provides a promising application with convenience and rapidness in the aptasensor fabrication for food safety analysis.

中文翻译：

基于RecJf核酸外切酶诱导的信号放大的新颖信号荧光on适传感器检测detection曲霉毒素A

本文介绍了一种用于检测homogeneous曲霉毒素A（OTA）的简单且均质的荧光适体传感器。具有高度的特异性和简单性；RecJ _˚F外切核酸酶用于切割FAM-适体/ OTA复合物的DNA链，并实现靶循环信号放大。为了避免反应系统的损失，在最后一个实验步骤中仅添加一次磁珠（MB）。提出的这种荧光适体传感器在0.1–100 ng / mL的范围内显示出更高的灵敏度，LOD为0.056 ng / mL，并且对其他干扰毒素具有良好的选择性。通过检测加标液体和谷物样品中的OTA，研究了制备的适体传感器的可行性。获得的平均回收率在92％至115％之间。这项研究为食品安全性分析在适体传感器的制造中提供了方便和快速的有希望的应用。

更新日期：2020-02-20

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