BLIMP1 (PRDM1) and VASA (DDX4) play pivotal roles in the development of the germ cell linage. Importantly, these genes are specifically expressed in germ cells; BLIMP1 in primordial germ cells (PGCs) to early-stage gonocytes, and VASA in migration-stage PGCs to mature gametes. The high reproductive efficiency of common marmosets (marmosets; Callithrix jacchus) makes them advantageous for use in germ cell research. We herein report the generation of a male marmoset embryonic stem cell (ESC) line harboring BLIMP1 and DDX4 double reporters. This ESC line will be a useful tool for investigating male gametogenesis in non-human primates.

BLIMP1 ( PRDM1 ) 和VASA ( DDX4 ) 在生殖细胞谱系的发育中起关键作用。重要的是，这些基因在生殖细胞中特异性表达。BLIMP1在原始生殖细胞 (PGCs) 到早期生殖母细胞中，VASA在迁移阶段 PGCs 到成熟配子中。普通狨猴（狨猴；  Callithrix jacchus）的高繁殖效率使其有利于生殖细胞研究。我们在此报告了含有BLIMP1和DDX4的雄性狨猴胚胎干细胞 (ESC) 系的产生双重记者。该 ESC 系将成为研究非人类灵长类动物雄性配子发生的有用工具。