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Paper-based electrodes modified with cobalt phthalocyanine colloid for the determination of hydrogen peroxide and glucose.
Analyst ( IF 3.6 ) Pub Date : 2020/02/20 , DOI: 10.1039/c9an02413a A Sánchez-Calvo 1 , A Costa-García , M C Blanco-López
Analyst ( IF 3.6 ) Pub Date : 2020/02/20 , DOI: 10.1039/c9an02413a A Sánchez-Calvo 1 , A Costa-García , M C Blanco-López
Affiliation
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Cobalt(ii) phthalocyanine (CoPc) was suspended in aqueous medium and the colloidal system was used as catalyst for the electrochemical determination of hydrogen peroxide on paper-based electrodes modified with carbon nanomaterials. H2O2 was oxidised at 0.275 V vs. Ag pseudoreference electrode. This system was adapted to develop a glucose sensor with glucose oxidase immobilized on the cellulose electrode. CoPc suspended nanoparticles acted as nanoenzyme mimicking peroxidase activity and were combined with different carbon nanomaterials to form hybrids with optimised catalytic performance. GO-CoPc paper-based electrodes yielded the best results with a linear range of ∼12 μM to 49 mM for H2O2 and 0.1 mM to 1 mM for glucose. Glucose was determined in physiological serum and juice samples with recoveries of 93.3 and 94.2% respectively. CoPc could replace HRP for the catalytic sensing of H2O2, without the need to be dissolved. This material can be used in situ in a simple protocol with other nanomaterials for electrode modification. The sensor described has the advantage of easy preparation, using the catalyst in colloidal form, long term stability, and versatility to be adapted to other low cost and disposable enzymatic systems.
中文翻译：
用酞菁钴胶体改性的纸基电极，用于测定过氧化氢和葡萄糖。
将酞菁钴（CoPc）悬浮在水性介质中，并将胶体体系用作催化剂，用碳纳米材料修饰的纸基电极对过氧化氢进行电化学测定。相对于Ag伪参比电极，H2O2在0.275 V下被氧化。该系统适于开发一种葡萄糖传感器，其中葡萄糖氧化酶固定在纤维素电极上。CoPc悬浮的纳米颗粒充当模仿过氧化物酶活性的纳米酶，并与不同的碳纳米材料结合形成具有优化催化性能的杂化体。GO-CoPc纸基电极产生的最佳结果是H2O2的线性范围为〜12μM至49 mM，葡萄糖的线性范围为〜0.1 mM至1 mM。测定生理血清和果汁样品中的葡萄糖，回收率分别为93.3％和94.2％。CoPc可以代替HRP进行H2O2的催化传感，而无需溶解。该材料可以与其他纳米材料以简单的协议原位使用，用于电极修饰。所描述的传感器的优点是易于制备，使用胶体形式的催化剂，长期的稳定性和通用性，以适应其他低成本和一次性酶体系。
更新日期：2020-03-31
中文翻译：
用酞菁钴胶体改性的纸基电极，用于测定过氧化氢和葡萄糖。
将酞菁钴（CoPc）悬浮在水性介质中，并将胶体体系用作催化剂，用碳纳米材料修饰的纸基电极对过氧化氢进行电化学测定。相对于Ag伪参比电极，H2O2在0.275 V下被氧化。该系统适于开发一种葡萄糖传感器，其中葡萄糖氧化酶固定在纤维素电极上。CoPc悬浮的纳米颗粒充当模仿过氧化物酶活性的纳米酶，并与不同的碳纳米材料结合形成具有优化催化性能的杂化体。GO-CoPc纸基电极产生的最佳结果是H2O2的线性范围为〜12μM至49 mM，葡萄糖的线性范围为〜0.1 mM至1 mM。测定生理血清和果汁样品中的葡萄糖，回收率分别为93.3％和94.2％。CoPc可以代替HRP进行H2O2的催化传感，而无需溶解。该材料可以与其他纳米材料以简单的协议原位使用，用于电极修饰。所描述的传感器的优点是易于制备，使用胶体形式的催化剂，长期的稳定性和通用性，以适应其他低成本和一次性酶体系。
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