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Avian Influenza Virus Detection by Optimized Peptide Termination on a Boron-Doped Diamond Electrode.
ACS Sensors ( IF 8.2 ) Pub Date : 2020-02-20 , DOI: 10.1021/acssensors.9b02126 Teruhiko Matsubara 1 , Michiko Ujie 1 , Takashi Yamamoto 2 , Yasuaki Einaga 2, 3 , Tomo Daidoji 4 , Takaaki Nakaya 4 , Toshinori Sato 1
ACS Sensors ( IF 8.2 ) Pub Date : 2020-02-20 , DOI: 10.1021/acssensors.9b02126 Teruhiko Matsubara 1 , Michiko Ujie 1 , Takashi Yamamoto 2 , Yasuaki Einaga 2, 3 , Tomo Daidoji 4 , Takaaki Nakaya 4 , Toshinori Sato 1
Affiliation
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The development of a simple detection method with high sensitivity is essential for the diagnosis and surveillance of infectious diseases. Previously, we constructed a sensitive biosensor for the detection of pathological human influenza viruses using a boron-doped diamond electrode terminated with a sialyloligosaccharide receptor-mimic peptide that could bind to hemagglutinins involved in viral infection. Circulation of influenza induced by the avian virus in humans has become a major public health concern, and methods for the detection of avian viruses are urgently needed. Here, peptide density and dendrimer generation terminated on the electrode altered the efficiency of viral binding to the electrode surface, thus significantly enhancing charge-transfer resistance measured by electrochemical impedance spectroscopy. The peptide-terminated electrodes exhibited an excellent detection limit of less than one plaque-forming unit of seasonal H1N1 and H3N2 viruses. Furthermore, the improved electrode was detectable for avian viruses isolated from H5N3, H7N1, and H9N2, showing the potential for the detection of all subtypes of influenza A virus, including new subtypes. The peptide-based electrochemical architecture provided a promising approach to biosensors for ultrasensitive detection of pathogenic microorganisms.
中文翻译：
通过在掺硼金刚石电极上优化的肽末端检测禽流感病毒。
开发具有高灵敏度的简单检测方法对于传染病的诊断和监视至关重要。以前，我们用硼掺杂的金刚石电极构建了一种灵敏的生物传感器，用于检测病理性人类流感病毒，该电极以可与涉及病毒感染的血凝素结合的唾液酸低聚糖受体模拟肽终止。由禽病毒在人类中传播的流感已成为主要的公共卫生问题，因此迫切需要检测禽病毒的方法。在此，终止于电极上的肽密度和树状聚合物的生成改变了病毒与电极表面结合的效率，从而显着增强了通过电化学阻抗谱测量的电荷转移阻力。肽终止电极显示出极好的检测限，不到季节性H1N1和H3N2病毒的一个噬菌斑形成单位。此外，改进的电极可检测到从H5N3，H7N1和H9N2分离出的禽病毒，显示出检测所有A型流感病毒亚型，包括新亚型的潜力。基于肽的电化学体系结构为生物传感器用于病原微生物的超灵敏检测提供了一种有前途的方法。
更新日期：2020-02-20
中文翻译：
通过在掺硼金刚石电极上优化的肽末端检测禽流感病毒。
开发具有高灵敏度的简单检测方法对于传染病的诊断和监视至关重要。以前，我们用硼掺杂的金刚石电极构建了一种灵敏的生物传感器，用于检测病理性人类流感病毒，该电极以可与涉及病毒感染的血凝素结合的唾液酸低聚糖受体模拟肽终止。由禽病毒在人类中传播的流感已成为主要的公共卫生问题，因此迫切需要检测禽病毒的方法。在此，终止于电极上的肽密度和树状聚合物的生成改变了病毒与电极表面结合的效率，从而显着增强了通过电化学阻抗谱测量的电荷转移阻力。肽终止电极显示出极好的检测限，不到季节性H1N1和H3N2病毒的一个噬菌斑形成单位。此外，改进的电极可检测到从H5N3，H7N1和H9N2分离出的禽病毒，显示出检测所有A型流感病毒亚型，包括新亚型的潜力。基于肽的电化学体系结构为生物传感器用于病原微生物的超灵敏检测提供了一种有前途的方法。
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