Restored interlaced volumetric imaging increases image quality and scanning speed during intravital imaging in living mice.,Journal of Biophotonics

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Restored interlaced volumetric imaging increases image quality and scanning speed during intravital imaging in living mice.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-03-02 , DOI: 10.1002/jbio.201960204
Maina Sogabe ₁ , Masayuki Ohzeki ₂ , Koji Fujimoto ₃ , Atsuko Sehara-Fujisawa ₁ , Satoshi Nishimura ₄

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Dynamic intravital imaging is essential for revealing ongoing biological phenomena within living organisms and is influenced primarily by several factors: motion artifacts, optical properties and spatial resolution. Conventional imaging quality within a volume, however, is degraded by involuntary movements and trades off between the imaged volume, imaging speed and quality. To balance such trade‐offs incurred by two‐photon excitation microscopy during intravital imaging, we developed a unique combination of interlaced scanning and a simple image restoration algorithm based on biological signal sparsity and a graph Laplacian matrix. This method increases the scanning speed by a factor of four for a field size of 212 μm × 106 μm × 130 μm, and significantly improves the quality of four‐dimensional dynamic volumetric data by preventing irregular artifacts due to the movement observed with conventional methods. Our data suggest this method is robust enough to be applied to multiple types of soft tissue.

中文翻译：

恢复的隔行体积成像可提高活体小鼠的活体内成像过程中的图像质量和扫描速度。

动态活体成像对于揭示活生物体内正在进行的生物学现象至关重要，并且主要受以下几个因素影响：运动伪影，光学特性和空间分辨率。但是，体积内的常规成像质量会因非自愿移动而降低，并在成像体积，成像速度和质量之间进行权衡。为了平衡在生物体内成像过程中双光子激发显微镜引起的这种折衷，我们开发了隔行扫描和基于生物信号稀疏性和图拉普拉斯矩阵的简单图像恢复算法的独特组合。对于212μm×106μm×130μm的场大小，此方法将扫描速度提高了四倍，并通过防止常规方法观察到的运动引起的不规则伪像，显着提高了三维动态体积数据的质量。我们的数据表明该方法足够鲁棒，可以应用于多种类型的软组织。

更新日期：2020-03-02

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