High-titer antibodies are a cause of false-negative reactions in red blood cell antigen phenotyping, an event referred to as blocked antigen phenomenon (BAP). In hemolytic disease of the fetus and newborn, BAP complicates laboratory workups as fetal phenotype is helpful in confirming the responsible antibody. Acid elution techniques, techniques using ethylenediaminetetraacetic glycine acid, as well as those using chloroquine diphosphate have been used to resolve BAP; however, ethylenediaminetetraacetic glycine acid destroys K-antigen expression and chloroquine diphosphate is not always effective. We report a case of severe hemolytic disease of the fetus and newborn from anti-K where a modified gentle heat elution resolved BAP. Although infrequently considered with isolated reports in the literature, heat elution is simple, is effective, and involves readily available materials in most blood banks.

高滴度抗体是红细胞抗原表型产生假阴性反应的原因，这一事件称为封闭抗原现象（BAP）。在胎儿和新生儿的溶血性疾病中，BAP使实验室检查复杂化，因为胎儿表型有助于确定负责任的抗体。酸洗脱技术，使用乙二胺四乙酸甘氨酸的技术以及使用氯喹二磷酸的技术已用于解决BAP；但是，乙二胺四乙酸甘氨酸会破坏K抗原的表达，而氯喹二磷酸并不总是有效的。我们报告了一例严重的胎儿和新生儿溶血性疾病的病例，该病例来自抗-K，改良的温和热洗脱可解决BAP。尽管文献中很少有单独的报告考虑使用热洗脱，但其简单，有效且涉及大多数血库中容易获得的材料。