In this study, haptens were designed to produce highly sensitive and specific monoclonal antibodies (mAb) against carbamazepine (CBZ) and its metabolite carbamazepine-10, 11-epoxide (CBZ-EP). According to the results of our competitive enzyme-linked immunosorbent assay (ic-ELISA), the half-maximum inhibitory concentration values for anti-CBZ and anti-CBZ-EP mAb were 0.18 and 0.59 ng/mL, respectively. An immunochromatographic assay (ICA) was developed for the determination of CBZ and CBZ-EP concentrations. This method can provide visible limits of detection ranging from 0.25 to 1 ng/mL, and cut-off limits ranging from 5 to 10 ng/mL, and takes 10 min to evaluate with the naked eye. Importantly, these observations were consistent with those obtained by ic-ELISA and liquid chromatography-mass spectrometry. The ICA assay represented a reliable, fast, and high-throughput method for the determination of CBZ and CBZ-EP in serum samples.

中文翻译：

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用于检测卡马西平和卡马西平-10，11-环氧化物的基于单克隆抗体的免疫色谱分析方法的开发。

在这项研究中，半抗原被设计为产生针对卡马西平（CBZ）及其代谢物卡马西平10，11-环氧化物（CBZ-EP）的高度敏感和特异的单克隆抗体（mAb）。根据我们竞争性酶联免疫吸附试验（ic-ELISA）的结果，抗CBZ和抗CBZ-EP mAb的半数最大抑制浓度值分别为0.18和0.59 ng / mL。开发了一种用于确定CBZ和CBZ-EP浓度的免疫色谱分析（ICA）。该方法可以提供0.25至1 ng / mL的可见检测限和5至10 ng / mL的截止限，并且用10分钟的时间进行肉眼评估。重要的是，这些观察结果与通过ic-ELISA和液相色谱-质谱法获得的观察结果一致。ICA分析代表了可靠，快速，