This work described the development of a novel method for simultaneous extraction of eight active compounds (including catechin, albiflorin, paeoniflorin, ferulic acid, ginsenoside Rg1, tetrahydropalmatine, ginsenoside Rb1 and osthole) from Yaobitong capsule by accelerated solvent extraction (ASE). Response surface methodology (RSM) with desirability functions was employed to optimize the extraction conditions yielding the optimal conditions of ASE (extraction time 8 min, extraction temperature 80 °C, extraction solvent 70% methanol and flushing volume 100%). A high-performance liquid chromatography coupled with a diode array detector (HPLC-DAD) method was developed and validated for simultaneous quantification of the eight compounds in Yaobitong capsule. The values of correlation coefficient (R) were satisfactory between 0.9992 and 0.9999 over the linear concentration range of 0.5-1000 μg mL-1. It was found that the limits of detection (LODs) and the limits of quantification (LOQs) for the eight active compounds were 0.10-2.90 μg•mL-1 and 0.30-9.40 μg•mL-1, respectively. The recoveries of the eight main active compounds in Yaobitong capsule were in the range of 93.31%-106.22%. And the contents of the analytes extracted by ASE under the optimal conditions were compared to traditional solvent extraction methods, such as ultrasonic and reflux extraction. The results indicated that the ASE method proved to be more suitable for the extract of active compounds in Yaobitong capsule, which could obtain higher extraction efficiency. At last, the proposed method was applied to analyze ten batches of actual samples, which provided high extraction efficiency and had wide potential application in the analysis of traditional Chinese medicines.

中文翻译：

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响应面分析法优化了腰必通胶囊中8种活性成分的加速溶剂萃取工艺：与超声和回流萃取的比较。

这项工作描述了一种通过加速溶剂萃取（ASE）同时从药必通胶囊中同时提取八种活性化合物（包括儿茶素，白花素，pa药苷，阿魏酸，人参皂苷Rg1，四氢巴马汀，人参皂苷Rb1和osthole）的新方法的开发。使用具有期望功能的响应面方法（RSM）来优化提取条件，从而获得ASE的最佳条件（提取时间8分钟，提取温度80°C，提取溶剂70％甲醇，冲洗量100％）。高效液相色谱结合二极管阵列检测器（HPLC-DAD）方法已开发出来，并经过验证可同时定量姚必通胶囊中的8种化合物。相关系数（R）的值在0.9992和0之间令人满意。线性浓度范围为0.5-1000μgmL-1时为9999。结果发现，这八种活性化合物的检出限（LOD）和定量限（LOQ）分别为0.10-2.90μg•mL-1和0.30-9.40μg•mL-1。腰必通胶囊中8种主要活性化合物的回收率在93.31％-106.22％之间。并将ASE在最佳条件下提取的分析物含量与传统的溶剂提取方法（如超声提取和回流提取）进行比较。结果表明，ASE法更适用于腰必通胶囊中活性成分的提取，提取效率更高。最后，将该方法应用于十批实际样品的分析，