Ganoderma lucidum is one of the most famous medicinal fungi and is traditional Chinese medicine with various biological activities in Asian countries. To clarify its pharmacodynamic material basis, 15 lanostane triterpenoidswere obtained from the fruiting bodies of G. lucidum, including 8 previously undescribed lanostanoids. Their structures, including absolute configuration, were established based on ultraviolet, infrared, high-resolution electrospray ionisation mass spectrometry, 1D and 2D nuclear magnetic resonance, and X-ray crystallographic analysis. Ganoluciduone A was an unusual octonorlanostane, which was isolated from Ganoderma for the first time. In addition, the anti-inflammatory activities of all isolates were evaluated by observing their inhibitory effects on nitric oxide production in RAW264.7 cells activated by a lipopolysaccharide. Ganoluciduone B exhibited moderate inhibitory activity on nitric oxide production, with an inhibition rate of 45.5% at a concentration of 12.5 μM.

中文翻译：

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来自灵芝的具有抗炎活性的羊毛脂烷三萜

灵芝是最著名的药用真菌之一，是亚洲国家具有多种生物活性的中药。为了阐明其药效物质基础，从灵芝子实体中提取了 15 种羊毛甾烷三萜，其中包括 8 种以前未描述过的羊毛甾烷。它们的结构，包括绝对构型，是基于紫外线、红外线、高分辨率电喷雾电离质谱、一维和二维核磁共振以及 X 射线晶体学分析建立的。Ganoluciduone A 是一种不寻常的辛诺兰甾烷，它是首次从灵芝中分离出来的。此外，通过观察它们对脂多糖激活的 RAW264.7 细胞中一氧化氮产生的抑制作用来评估所有分离物的抗炎活性。