Dental implants are very successful medical devices, yet implant failures do occur due to biological and mechanical complications. Peri-implantitis is one such biological complication that is primarily caused by bacteria and their products at the implant soft tissue interface. Bacterial infiltration can be prevented by the formation of a reliable soft tissue seal encircling dental implants. Platelet-derived growth factor-BB (PDGF-BB) has significant chemotactic and proliferative effects on various mesenchymal cell types, including fibroblasts, and therefore can be an effective molecule to enhance the peri-implant soft tissue seal. To overcome the limitations of the recombinant protein form of PDGF-BB, such as cost and the need for supraphysiological doses, we have developed and characterized a titanium surface that is rendered bioactive by coating it with polyethylenimine-plasmid DNA (pDNA) nanoplexes in the presence of sucrose. Human embryonic kidney 293T (HEK293T) cells and human primary gingival fibroblasts (GFs) were successfully transfected in culture with enhanced green fluorescent protein (EGFP)-encoding pDNA or platelet-derived growth factor subunit B (PDGFB)-encoding pDNA loaded into nanoplexes and coated onto titanium disks in a dose-dependent manner. GFs were shown to secrete PDGF-BB for at least 7 days after transfection and displayed both minimal viability loss and increased integrin-α2 expression 4 days posttransfection.

中文翻译：

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用于口腔种植的概念基因激活钛表面的证明。

牙科植入物是非常成功的医疗设备，但是由于生物学和机械并发症，植入物确实会发生故障。种植体周围炎是一种这样的生物并发症，主要由细菌及其在植入物软组织界面处的产物引起。可以通过围绕牙植入物形成可靠的软组织密封来防止细菌浸润。血小板衍生生长因子-BB（PDGF-BB）对各种间充质细胞（包括成纤维细胞）具有显着的趋化和增殖作用，因此可以作为增强植入物周围软组织密封的有效分子。为了克服PDGF-BB重组蛋白形式的局限性，例如成本和超生理剂量的需要，我们已经开发并鉴定了钛表面，该表面在蔗糖存在的情况下通过用聚乙烯亚胺质粒DNA（pDNA）纳米复合物包被使其具有生物活性。用增强的绿色荧光蛋白（EGFP）编码pDNA或血小板衍生的生长因子亚基B（PDGFB）编码的pDNA成功地将人类胚胎肾293T（HEK293T）细胞和人类原发性牙龈成纤维细胞（GFs）转染到培养物中，并成功地将其转染到纳米复合体中。以剂量依赖的方式涂在钛盘上。转染后至少7天，GFs会分泌PDGF-BB，转染后4天，GFs的活力丧失最小，整联蛋白-α2表达增加。用增强的绿色荧光蛋白（EGFP）编码pDNA或血小板衍生的生长因子亚基B（PDGFB）编码的pDNA成功地将人类胚胎肾293T（HEK293T）细胞和人类原发性牙龈成纤维细胞（GFs）转染到培养物中，并成功地将其转染到纳米复合体中。以剂量依赖的方式涂在钛盘上。转染后至少7天，GFs会分泌PDGF-BB，转染后4天，GFs的活力丧失最小，整联蛋白-α2表达增加。用增强的绿色荧光蛋白（EGFP）编码pDNA或血小板衍生的生长因子亚基B（PDGFB）编码的pDNA成功地将人类胚胎肾293T（HEK293T）细胞和人类原发性牙龈成纤维细胞（GFs）转染到培养物中，并成功地将其转染到纳米复合体中。以剂量依赖的方式涂在钛盘上。转染后至少7天，GFs会分泌PDGF-BB，转染后4天，GFs的活力丧失最小，整联蛋白-α2表达增加。