当前位置：
X-MOL 学术
›
Bioelectrochemistry
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Polymer/enzyme-modified HF-etched carbon nanoelectrodes for single-cell analysis.
Bioelectrochemistry ( IF 4.8 ) Pub Date : 2020-02-19 , DOI: 10.1016/j.bioelechem.2020.107487 Miriam Marquitan 1 , Adrian Ruff 1 , Mattia Bramini 2 , Stefan Herlitze 3 , Melanie D Mark 3 , Wolfgang Schuhmann 1
Bioelectrochemistry ( IF 4.8 ) Pub Date : 2020-02-19 , DOI: 10.1016/j.bioelechem.2020.107487 Miriam Marquitan 1 , Adrian Ruff 1 , Mattia Bramini 2 , Stefan Herlitze 3 , Melanie D Mark 3 , Wolfgang Schuhmann 1
Affiliation
|
Carbon-based nanoelectrodes fabricated by means of pyrolysis of an alkane precursor gas purged through a glass capillary and subsequently etched with HF were modified with redox polymer/enzyme films for the detection of glucose at the single-cell level. Glucose oxidase (GOx) was immobilized and electrically wired by means of an Os-complex-modified redox polymer in a sequential dip coating process. For the synthesis of the redox polymer matrix, a poly(1-vinylimidazole-co-acrylamide)-based backbone was used that was first modified with the electron transfer mediator [Os(bpy)2Cl]+ (bpy = 2,2'-bipyridine) followed by the conversion of the amide groups within the acrylamide monomer into hydrazide groups in a polymer-analogue reaction. The hydrazide groups react readily with bifunctional epoxide-based crosslinkers ensuring high film stability. Insertion of the nanometre-sized polymer/enzyme modified electrodes into adherently growing single NG108-15 cells resulted in a positive current response correlating with the intracellular glucose concentration. Moreover, the nanosensors showed a stable current output without significant loss in performance after intracellular measurements.
中文翻译：
用于单细胞分析的聚合物/酶修饰的HF蚀刻碳纳米电极。
通过氧化还原聚合物/酶膜对通过玻璃毛细管吹扫并随后用HF蚀刻的烷烃前体气体进行热解制得的碳基纳米电极进行修饰，以检测单细胞水平的葡萄糖。葡萄糖氧化酶（GOx）在连续浸涂过程中通过Os-络合物改性的氧化还原聚合物固定并电连接。为了合成氧化还原聚合物基质，使用了基于聚（1-乙烯基咪唑-丙烯酰胺）的骨架，该骨架首先用电子转移介体[Os（bpy）2Cl] +（bpy = 2,2'-联吡啶），然后在聚合物类似物反应中将丙烯酰胺单体中的酰胺基转化为酰肼基。酰肼基易于与基于双官能团环氧化物的交联剂反应，从而确保了高膜稳定性。纳米大小的聚合物/酶修饰电极插入到粘附生长的单个NG108-15细胞中会产生与细胞内葡萄糖浓度相关的正电流响应。此外，纳米传感器在细胞内测量后显示稳定的电流输出，而性能没有明显损失。
更新日期：2020-02-20
中文翻译：
用于单细胞分析的聚合物/酶修饰的HF蚀刻碳纳米电极。
通过氧化还原聚合物/酶膜对通过玻璃毛细管吹扫并随后用HF蚀刻的烷烃前体气体进行热解制得的碳基纳米电极进行修饰，以检测单细胞水平的葡萄糖。葡萄糖氧化酶（GOx）在连续浸涂过程中通过Os-络合物改性的氧化还原聚合物固定并电连接。为了合成氧化还原聚合物基质，使用了基于聚（1-乙烯基咪唑-丙烯酰胺）的骨架，该骨架首先用电子转移介体[Os（bpy）2Cl] +（bpy = 2,2'-联吡啶），然后在聚合物类似物反应中将丙烯酰胺单体中的酰胺基转化为酰肼基。酰肼基易于与基于双官能团环氧化物的交联剂反应，从而确保了高膜稳定性。纳米大小的聚合物/酶修饰电极插入到粘附生长的单个NG108-15细胞中会产生与细胞内葡萄糖浓度相关的正电流响应。此外，纳米传感器在细胞内测量后显示稳定的电流输出，而性能没有明显损失。
京公网安备 11010802027423号