Articular cartilage defects are one of the major challenges in orthopedic and trauma surgery. However, the poor ability of cartilage to self-repair has motivated efforts to engineer replacement tissues, and human mesenchymal stem cells (MSC), which have an extensive proliferation potential and can undergo chondrogenesis, have emerged as a promising cell source. In this review, we attempt to provide a brief overview of MSC isolation, characterization, current manufacturing platforms using various bioreactors, in vitro differentiation, and sealant-based or scaffold-based implantation.

中文翻译：

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人类间充质干细胞疗法用于软骨修复：关于分离，扩增和构建的评论。

关节软骨缺损是整形外科和创伤外科手术的主要挑战之一。然而，软骨自修复的能力差促使人们努力设计替代组织，并且具有广泛增殖潜能并可以进行软骨形成的人间充质干细胞（MSC）已成为有希望的细胞来源。在这篇综述中，我们试图简要概述MSC的分离，表征，使用各种生物反应器的当前制造平台，体外分化以及基于密封剂或基于支架的植入。