Plant tissue culture is a highly versatile technique which leads to micropropagation of thousands of plantlets from a small portion of the mother plant in vitro. This review for the first time has addressed and presented all the accomplishments made till date through the use of mass spectrometry for plant tissue culture analysis. The achievements enabled through the fusion of these distinctly different technologies in the area of MS based proteomics and MS based metabolomics and secretomics have been elaborated. The lacunae in not employing the use of the recently evolved MS techniques especially the imaging MS technique is highlighted. If merely applying traditional forms of MS could bring about the achievements reported, then the use of the state-of-the-art sophistications in MS is proposed to yield greater breakthroughs.

植物组织培养是一种用途广泛的技术，可从一小部分母本植物中体外繁殖成千上万株小植株。这篇综述首次解决并介绍了迄今为止通过使用质谱进行植物组织培养分析而取得的所有成就。阐述了通过融合基于MS的蛋白质组学和基于MS的代谢组学和分泌组学领域的这些截然不同的技术所取得的成就。突出显示了不使用最近发展的MS技术（尤其是成像MS技术）的缺陷。如果仅采用传统形式的MS可以带来所报告的成就，那么建议在MS中使用最先进的技术以取得更大的突破。