Incorporation of Indole Significantly Improves the Transfection Efficiency of Guanidinium-Containing Poly(Methacrylamide)s.,Macromolecular Rapid Communications

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Incorporation of Indole Significantly Improves the Transfection Efficiency of Guanidinium-Containing Poly(Methacrylamide)s.
Macromolecular Rapid Communications ( IF 4.2 ) Pub Date : 2020-02-19 , DOI: 10.1002/marc.201900668
Ceren Cokca ₁ , Leon Zartner ₂ , Ilja Tabujew ₁ , Dagmar Fischer _{2,

3} , Kalina Peneva _{1,

3}

Affiliation

A highly efficient transfection agent is reported that is based on terpolymer consisting of N-(2-hydroxypropyl)methacrylamide (HPMA), N-(3-guanidinopropyl) methacrylamide (GPMA), and N-(2-indolethyl)methacrylamide monomers (IEMA) by analogy to the amphipathic cell-penetrating peptides containing tryptophan and arginine residues. The incorporation of the indole-bearing monomer leads to successful plasmid DNA condensation even at a nitrogen-to-phosphate (N/P) ratio of 1. The hydrodynamic diameter of polyplexes is determined to be below 200 nm for all N/P ratios. The transfection studies demonstrate a 200-fold increase of the transgene expression in comparison to P(HPMA-co-GPMA) with the same guanidinium content. This study reveals the strong potential of the indole group as a side-chain pendant group that can increase the cellular uptake of polymers and the transfection efficiency of the respective polyplexes.

中文翻译：

吲哚的加入显着提高了含胍基聚甲基丙烯酰胺的转染效率。

据报道，一种高效转染剂是基于由N-（2-羟丙基）甲基丙烯酰胺（HPMA），N-（3-胍基丙基）甲基丙烯酰胺（GPMA）和N-（2-吲哚乙基）甲基丙烯酰胺单体（IEMA）构成的三元共聚物类似于含有色氨酸和精氨酸残基的两亲性可穿透细胞的肽。即使在氮磷比（N / P）为1的情况下，带有吲哚的单体的掺入也会成功地使质粒DNA缩合。对于所有N / P比率，测得的复合物的流体动力学直径均低于200 nm。转染研究表明，与具有相同胍盐含量的P（HPMA-co-GPMA）相比，转基因表达增加了200倍。

更新日期：2020-02-20

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