Acne is a common chronic inflammatory skin disease, and the inflammation immune response runs through all stages of acne lesions. In this study, we use a combination of multiplex-PCR and high-throughput sequencing technologies to analyze T cell receptor β chain CDR3 (complementarity-determining region 3) in peripheral blood isolated from severe acne patients. Once compared with healthy controls, we propose to identify acne-relevant CDR3 peptides. Our results reveal that the diversity of T cell receptor β chain (TRB) CDR3 sequences in the peripheral blood of the severe acne vulgaris (SA) group differed from that of the control group. In addition, we find 10 TRB CDR3 sequences, amino acid sequences and V-J combinations with significantly different expressions between the SA group and the non-acne (NA) group (P < 0.0001). These findings may contribute to a better understanding of the role of immunity in the pathogenesis of acne and may serve as biomarkers for evaluating risk or prognosis of severe acne disease in future.

中文翻译：

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高通量测序揭示了重症痤疮患者中TCRβ链CDR3的多样性。

痤疮是一种常见的慢性炎症性皮肤病，炎症免疫反应贯穿痤疮病变的各个阶段。在这项研究中，我们使用多重PCR和高通量测序技术的组合来分析严重痤疮患者外周血中的T细胞受体β链CDR3（互补决定区3）。与健康对照组比较后，我们建议鉴定痤疮相关的CDR3肽。我们的结果表明，重度寻常痤疮（SA）组外周血中T细胞受体β链（TRB）CDR3序列的多样性与对照组不同。此外，我们发现10个TRB CDR3序列，氨基酸序列和VJ组合在SA组和非痤疮（NA）组之间的表达明显不同（P <0.0001）。