Abstract Scaffolds consisting of cylindrical struts are one of the high-potential tissue engineering materials because the highly porous structure can easily induce cell infiltration/migration and efficiently deliver nutrients to the cells. In addition, cryopreservable scaffolds have attracted much interest in tissue engineering because they can be prospective ready-to-use “living” biomaterials consisting of a patient’s own cells. In this study, we investigated a cryopreservable cell-printed scaffold consisting of microscale cylindrical struts. To fabricate the scaffold, we developed a 3D cell-printing system supplemented with microfluidic channels, a core-shell nozzle, UV treatment system, and low-temperature working plate. The scaffold consisted of a cell-laden collagen/dimethyl sulfoxide (DMSO) mixture in the core region and a methacrylate gelatin (GelMA)/DMSO mixture in the shell region. After cryopreservation, the preosteoblasts (MC3T3-E1) loaded in the scaffold showed reasonable cell viability (∼85%). Moreover, no significant difference was observed in the cell proliferations and ALP activities of the cryopreserved scaffold and non-cryopreserved scaffold. Based on these results, we believe that the fabrication process can be one of the potential techniques for fabricating cryopreservable scaffolds consisting of cylindrical struts.

中文翻译：

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使用 3D 打印工艺制造的可冷冻保存的载有细胞的 GelMa 支架，并辅以原位光交联

摘要 由圆柱支柱组成的支架是一种高潜力的组织工程材料，因为其高度多孔的结构可以很容易地诱导细胞浸润/迁移并有效地向细胞输送营养。此外，可冷冻保存的支架在组织工程中引起了极大的兴趣，因为它们可以是由患者自身细胞组成的预期即用型“活”生物材料。在这项研究中，我们研究了一种由微型圆柱支柱组成的可冷冻保存的细胞打印支架。为了制造支架，我们开发了一个 3D 细胞打印系统，辅以微流体通道、核壳喷嘴、紫外线处理系统和低温工作板。支架由核心区域的细胞负载胶原蛋白/二甲基亚砜 (DMSO) 混合物和壳区域的甲基丙烯酸明胶 (GelMA)/DMSO 混合物组成。冷冻保存后，支架中装载的前成骨细胞（MC3T3-E1）显示出合理的细胞活力（~85%）。此外，在冻存支架和非冻存支架的细胞增殖和 ALP 活性方面​​没有观察到显着差异。基于这些结果，我们相信制造过程可以成为制造由圆柱形支柱组成的可低温保存的支架的潜在技术之一。冻存支架和非冻存支架的细胞增殖和ALP活性未观察到显着差异。基于这些结果，我们相信制造过程可以成为制造由圆柱形支柱组成的可低温保存的支架的潜在技术之一。冻存支架和非冻存支架的细胞增殖和ALP活性未观察到显着差异。基于这些结果，我们相信制造过程可以成为制造由圆柱形支柱组成的可低温保存的支架的潜在技术之一。