当前位置：
X-MOL 学术
›
Pestic. Biochem. Phys.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Antibacterial mode of action of trans-cinnamaldehyde derived from cinnamon bark (Cinnamomum verum) essential oil against Agrobacterium tumefaciens
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.pestbp.2020.02.012 Ji-Eun Lee 1 , Myunghee Jung 2 , Sung-Chan Lee 3 , Min-Jung Huh 3 , Seon-Mi Seo 4 , Il-Kwon Park 4
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.pestbp.2020.02.012 Ji-Eun Lee 1 , Myunghee Jung 2 , Sung-Chan Lee 3 , Min-Jung Huh 3 , Seon-Mi Seo 4 , Il-Kwon Park 4
Affiliation
|
The fumigant antibacterial activities of 50 plant essential oils belonging to 10 families were investigated against Agrobacterium tumefaciens. Among the test plant essential oils, Cinnamomum verum (cinnamon bark) essential oil showed the most potent fumigant antibacterial activity. When we investigated the antibacterial activities of compounds identified from cinnamon bark essential oil and 9 congeners of trans-cinnamaldehyde, lengths of inhibition zone of trans-cinnamaldehyde, salicylaldehyde and hydrocinnamaldehyde were 1.28, 1.73, and 1.24 cm at 0.625 mg/paper disc concentration, respectively. To determine the mode of action of trans-cinnamaldehyde and salicylaldehyde, intercellular reactive oxygen species (ROS) generation and cell membrane integrity were determined using a confocal laser scanning microscopy. Furthermore, we compared the up- and down-regulated gene expression of A. tumefaciens treated with trans-cinnamaldehyde and salicylaldehyde with that of untreated A. tumefaciens. With cutoffs of |log2FC| > 1 and FDR < 0.05, 29 and 43 down-regulated genes and 27 and 117 up-regulated genes were found in the treatment of trans-cinnamaldehyde and salicylaldehyde, respectively. Based on the ROS generation results, cell membrane integrity assay, and gene expression, we conclude that the antibacterial mode of action of trans-cinnamaldehyde and salicylaldehyde is ROS generation by the Fenton reaction caused by the down-regulation of an ATP synthesis-related gene cluster, corrupted iron ion homeostasis, and a corrupted ROS defense mechanism. The high concentration of ROS damaged the A. tumefaciens cell membrane, which caused cell death.
中文翻译：
来自肉桂皮（Cinnamomum verum）精油的反式肉桂醛对根癌农杆菌的抗菌作用方式
研究了10科50种植物精油对根癌农杆菌的熏蒸抗菌活性。在测试植物精油中，Cinnamomum verum（肉桂皮）精油显示出最有效的熏蒸抗菌活性。当我们研究从肉桂皮精油和 9 种反式肉桂醛同系物中鉴定的化合物的抗菌活性时，反式肉桂醛、水杨醛和氢化肉桂醛的抑菌圈长度在 0.625 mg/纸盘浓度下分别为 1.28、1.73 和 1.24 cm，分别。为了确定反式肉桂醛和水杨醛的作用模式，使用共聚焦激光扫描显微镜确定细胞间活性氧 (ROS) 的生成和细胞膜完整性。此外，我们比较了用反式肉桂醛和水杨醛处理的根癌农杆菌的上调和下调基因表达与未处理的根癌农杆菌的基因表达。具有 |log2FC| 的截止值 > 1 和 FDR < 0.05，分别在反式肉桂醛和水杨醛处理中发现了 29 和 43 个下调基因以及 27 个和 117 个上调基因。基于 ROS 生成结果、细胞膜完整性测定和基因表达，我们得出结论，反式肉桂醛和水杨醛的抗菌作用模式是由 ATP 合成相关基因下调引起的芬顿反应产生 ROS。簇、损坏的铁离子稳态和损坏的 ROS 防御机制。高浓度的 ROS 会破坏 A. tumefaciens 细胞膜，导致细胞死亡。
更新日期：2020-05-01
中文翻译：
来自肉桂皮（Cinnamomum verum）精油的反式肉桂醛对根癌农杆菌的抗菌作用方式
研究了10科50种植物精油对根癌农杆菌的熏蒸抗菌活性。在测试植物精油中，Cinnamomum verum（肉桂皮）精油显示出最有效的熏蒸抗菌活性。当我们研究从肉桂皮精油和 9 种反式肉桂醛同系物中鉴定的化合物的抗菌活性时，反式肉桂醛、水杨醛和氢化肉桂醛的抑菌圈长度在 0.625 mg/纸盘浓度下分别为 1.28、1.73 和 1.24 cm，分别。为了确定反式肉桂醛和水杨醛的作用模式，使用共聚焦激光扫描显微镜确定细胞间活性氧 (ROS) 的生成和细胞膜完整性。此外，我们比较了用反式肉桂醛和水杨醛处理的根癌农杆菌的上调和下调基因表达与未处理的根癌农杆菌的基因表达。具有 |log2FC| 的截止值 > 1 和 FDR < 0.05，分别在反式肉桂醛和水杨醛处理中发现了 29 和 43 个下调基因以及 27 个和 117 个上调基因。基于 ROS 生成结果、细胞膜完整性测定和基因表达，我们得出结论，反式肉桂醛和水杨醛的抗菌作用模式是由 ATP 合成相关基因下调引起的芬顿反应产生 ROS。簇、损坏的铁离子稳态和损坏的 ROS 防御机制。高浓度的 ROS 会破坏 A. tumefaciens 细胞膜，导致细胞死亡。
京公网安备 11010802027423号