Abstract Hericium novae-zealandiae is a native mushroom consumed by indigenous Māori people in New Zealand. The lipophilic mycochemicals of the mushroom were isolated using a normal column chromatography combined with a preparative HPLC. Structural characterisation based on spectroscopic methods, namely UV, MS, NMR and single crystal XRD have identified three lipophilic compounds as hericene B (a compound unique to Hericium), ergosterol and ergosterol peroxide. Following this, an HPLC-DAD method was developed and validated to quantify the hericene B and ergosterol. The method showed excellent selectivity, linearity, precision, accuracy and robustness. The content of hericene B was determined as 28.53 mg/g by dry weight of H. novae-zealandiae (approximately 3%). This discovery indicates the potential utilisation of H. novae-zealandiae as a natural source of hericene B. Current research revealed for the first time, the lipophilic constituents of H. novae-zealandiae and the method development for quantification of hericene B in the above species.

中文翻译：

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新西兰天然食用菌Hericium novae-zealandiae亲脂性真菌化学物质的分离与测定

摘要 Hericium novae-zealandiae 是新西兰土著毛利人食用的一种本地蘑菇。使用常规柱色谱结合制备型 HPLC 分离蘑菇的亲脂性真菌化学物质。基于光谱方法，即 UV、MS、NMR 和单晶 XRD 的结构表征已确定了三种亲脂性化合物为 hericene B（猴头菇特有的化合物）、麦角甾醇和过氧化麦角甾醇。在此之后，开发并验证了一种 HPLC-DAD 方法来量化 hericene B 和麦角甾醇。该方法显示出优异的选择性、线性、精密度、准确度和稳健性。以新西兰花干重计，hericene B 的含量测定为 28.53 mg/g（约 3%）。这一发现表明 H.