The aim of this study is a present of a simple solvothermal synthesis approach to preparation of Cu-based magnetic metal organic framework (MMOF) and subsequently its application as sorbent for ultrasound assisted magnetic solid phase extraction (UAMSPE) of ampicillin (AMP) from cow milk samples prior to high performance liquid chromatography-Ultraviolet (HPLC-UV) determination. Characteristics of prepared MMOF were fully investigated by different techniques which showed the exclusive properties of proposed sorbent in terms of proper functionality, desirable magnetic property and also high specific surface area. Different influential factors on extraction recovery including sorbent dosage, ultrasonic time, washing solvent volume and eluent solvent volume were assessed using central composite design (CCD) based response surface methodology (RSM) as an operative and powerful optimization tool. This is the first report for determination of AMP using MMOF. The proposed method addressed some drawbacks of other methods and sorbents for determination of AMP. The presented method decreases the extraction time (4 min) and also enhances adsorption capacity (250 mg/g). Moreover, the magnetic property of presented sorbent (15 emu/g) accelerates the extraction process which does not need filtration, centrifuge and precipitation procedures. Under the optimized conditions, the proposed method is applicable for linear range of 1.0–5000.0 μg/L with detection limit of 0.29 μg/L, satisfactory recoveries (≥95.0%) and acceptable repeatability (RSD less than 4.0%). The present study indicates highly promising perspectives of MMOF for highly effective analysis of AMP in complicated matrices.

本研究的目的是介绍一种简单的溶剂热合成方法来制备铜基磁性金属有机骨架 (MMOF)，并随后将其作为吸附剂用于牛氨苄青霉素 (AMP) 的超声辅助磁性固相萃取 (UAMSPE)高效液相色谱-紫外 (HPLC-UV) 测定之前的牛奶样品。通过不同的技术对制备的 MMOF 的特性进行了充分研究，这些技术显示了所提出的吸附剂在适当的功能、理想的磁性和高比表面积方面的独特性能。不同影响萃取回收率的因素包括吸附剂用量、超声时间、使用基于中心复合设计 (CCD) 的响应面法 (RSM) 作为有效且强大的优化工具，评估洗涤溶剂体积和洗脱溶剂体积。这是使用 MMOF 测定 AMP 的第一份报告。所提出的方法解决了用于测定 AMP 的其他方法和吸附剂的一些缺点。所提出的方法减少了提取时间（4 分钟），也提高了吸附能力（250 毫克/克）。此外，所提出的吸附剂 (15 emu/g) 的磁性加速了不需要过滤、离心和沉淀程序的提取过程。在优化条件下，该方法适用于线性范围1.0~5000.0 μg/L，检出限为0.29 μg/L，回收率满意（≥95.0%），重复性可接受（RSD小于4.0%）。