In the past decade, dried blood spot (DBS) sampling has been used increasingly for microsampling in various fields. This is predominantly driven by the significant advantages DBS offers regarding simple sample retrieval and shipment, combined with increased analyte stability. However, the manual handling of DBS samples is laborsome and prevents the use of a high-capacity bioanalytical workflow. The recent introduction of robotic DBS extraction systems in combination with liquid chromatography-tandem mass spectrometry (LC-MS/MS) has enabled the full automation of the analytical process. This results in overall higher sample throughput, minimal user interaction, and a significant reduction in consumables. Different instrumental setups are currently available which differ with respect to the extraction process, extract processing strategy, and internal standard application. This review article provides an overview of fully automated DBS analysis for one of these instruments, the DBS-MS 500 autosampler from CAMAG.

中文翻译：

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使用 CAMAG DBS-MS 500 自动进样器进行全自动干血斑分析在液相色谱-串联质谱法中的应用

在过去的十年中，干血点（DBS）采样越来越多地用于各个领域的微量采样。这主要是由 DBS 在简单的样品检索和运输方面提供的显着优势以及更高的分析物稳定性推动的。然而，DBS 样品的手动处理非常费力，并且妨碍了高容量生物分析工作流程的使用。最近推出的机器人 DBS 萃取系统与液相色谱-串联质谱 (LC-MS/MS) 相结合，实现了分析过程的完全自动化。这导致总体上更高的样品通量、最少的用户交互以及显着减少的耗材。目前有不同的仪器设置可供选择，这些设置在提取过程、提取物处理策略和内标应用方面有所不同。本文概述了其中一种仪器（CAMAG 的 DBS-MS 500 自动进样器）的全自动 DBS 分析。