Despite best efforts to optimize reproduction, egg incubation, and larval performance in captivity, inconsistencies in hatchery fish production are still created by high variations in egg quality from individual females. In some hatchery species, egg quality and generation of viable embryos are correlated to abundances of specific mRNAs. Channel catfish females show considerable extremes in egg quality, causing inconsistencies in channel catfish, Ictalurus punctatus, female × blue catfish, Ictalurus furcatus, male hybrid fry production. The objectives of this study were to examine relative transcripts linked to egg and embryo quality and determine expression between low-hatch and high-hatch egg batches through early development (0, 24, 48, and 96 h post-fertilization; HPF). RNA was extracted from eggs/embryos of nine females (n = 4 high-quality, n = 5 low-quality) and Real-Time PCR was used to quantify relative gene expression. The transcripts assessed in this study perform critical cellular functions, including tubulin β (tubb), cathepsin D (ctsd), cathepsin Z (ctsz), cathepsin B (ctsb), cyclin B (ccnb1), exportin-1 (xpo1), ring finger protein 213 (rnf213), glucocorticoid receptor-1 (GR-1), and heat shock protein 70 (hsp70). Relative gene expression of all transcripts except GR-1 and hsp70 were up-regulated in the high-hatch group and peaked at 48 HPF (neurulation stage), indicating the importance of these gene products at this threshold to normally progress until hatch. Due to lack of expression during earlier stages, maternally derived mRNAs for these genes do not seem to impact early embryonic development. Using mRNA markers as a selection mechanism for hatchery broodstock may lead to more high-hatch egg batches by reducing problems associated with poor egg quality.

中文翻译：

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分析特定mRNA基因表达谱作为杂交hybrid鱼水产养殖卵和胚胎质量的标志。

尽管尽了最大的努力来优化繁殖，卵的孵化和圈养的幼体性能，但由于雌鱼卵质量的高度差异，孵化场鱼类生产仍存在不一致之处。在某些孵化场物种中，卵的质量和有活力的胚胎的产生与特定mRNA的丰度相关。鱼雌性在鸡蛋品质上表现出极大的极端性，从而导致了cat鱼，Ictalurus punctatus，雌性×蓝色cat鱼，Ictalurus furcatus，雄性杂种鱼苗的不一致。这项研究的目的是检查与卵和胚胎质量有关的相对转录本，并通过早期发育（受精后0、24、48和96 h； HPF）确定低孵化率和高孵化率的卵之间的表达。从9位雌性的卵/胚中提取RNA（n = 4个高质量，n = 5低质量），并使用实时PCR定量相对基因表达。在这项研究中评估的转录本执行关键的细胞功能，包括微管蛋白β（tubb），组织蛋白酶D（ctsd），组织蛋白酶Z（ctsz），组织蛋白酶B（ctsb），细胞周期蛋白B（ccnb1），输出蛋白1（xpo1），环手指蛋白213（rnf213），糖皮质激素受体1（GR-1）和热休克蛋白70（hsp70）。在高孵化组中，除GR-1和hsp70以外的所有转录本的相对基因表达均被上调，并在48 HPF（培养阶段）达到峰值，表明这些基因产物在此阈值下对正常孵化直至孵化的重要性。由于在早期阶段缺乏表达，这些基因的母体来源的mRNA似乎不会影响早期的胚胎发育。