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Aryl hydrocarbon receptor nuclear translocators (ARNT1, ARNT2, and ARNT3) of white sturgeon (Acipenser transmontanus): Sequences, tissue-specific expressions, and response to β-naphthoflavone.
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2020-02-17 , DOI: 10.1016/j.cbpc.2020.108726 Jon A Doering 1 , Shawn C Beitel 2 , Sarah Patterson 2 , Bryanna K Eisner 2 , John P Giesy 3 , Markus Hecker 4 , Steve Wiseman 5
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2020-02-17 , DOI: 10.1016/j.cbpc.2020.108726 Jon A Doering 1 , Shawn C Beitel 2 , Sarah Patterson 2 , Bryanna K Eisner 2 , John P Giesy 3 , Markus Hecker 4 , Steve Wiseman 5
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Sturgeons (Acipenseridae) are ancient fishes that have tissue-specific profiles of transcriptional responses to dioxin-like compounds (DLCs) that are unique from those generally measured in teleost fishes. Because DLCs exert their critical toxicities through activation of the aryl hydrocarbon receptor (AHR), this transcription factor has been the subject of intensive study. However, less attention has focused on the aryl hydrocarbon receptor nuclear translocator (ARNT), which is the dimerization partner of the AHR and required for AHR-mediated transcription. The present study sequenced ARNT1, ARNT2, and ARNT3 in a representative species of sturgeon, the white sturgeon (Acipenser transmontanus), and quantified tissue-specific basal transcript abundance for each ARNT and the response following exposure to the model agonist of the AHR, β-naphthoflavone. In common with other proteins in sturgeons, the amino acid sequences of ARNTs are more similar to those of tetrapods than are ARNTs of other fishes. Transcripts of ARNT1, ARNT2, and ARNT3 were detected in all tissues investigated. Expression of ARNTs are tightly regulated in vertebrates, but β-naphthoflavone caused down-regulation in liver and up-regulation in gill, while an upward trend was measured in intestine. ARNTs are dimeric partners for multiple proteins, including the hypoxia inducible factor 1α (HIF1α), which mediates response to hypoxia. A downward trend in abundance of HIF1α transcript was measured in liver of white sturgeon exposed to β-naphthoflavone. Altered expression of ARNTs and HIF1α caused by activation of the AHR might affect the ability of certain tissues in sturgeons to respond to hypoxia when co-exposed to DLCs or other agonists.
中文翻译：
白色st鱼（Acipenser transmontanus）的芳烃受体核转运子（ARNT1，ARNT2和ARNT3）：序列，组织特异性表达和对β-萘黄酮的反应。
urge鱼（Acipenseridae）是古老的鱼类，具有对二恶英样化合物（DLCs）的转录反应的组织特异性特征，这与硬骨鱼类中的一般特征不同。由于DLC通过激活芳烃受体（AHR）发挥其关键毒性，因此该转录因子已成为深入研究的主题。但是，较少的注意力集中在芳烃受体核转运子（ARNT）上，它是AHR的二聚体伴侣，是AHR介导的转录所必需的。本研究对代表性a鱼，白色st鱼（Acipenser transmontanus）中的ARNT1，ARNT2和ARNT3进行了测序，并对每个ARNT的组织特异性基础转录本丰度和暴露于AHR模型激动剂后的响应进行了定量分析，β-萘黄酮。与st鱼中的其他蛋白质相同，ARNTs的氨基酸序列与四足动物的氨基酸序列比其他鱼类的ARNTs更为相似。在所有调查的组织中均检测到ARNT1，ARNT2和ARNT3的转录本。在脊椎动物中，ARNTs的表达受到严格的调节，但是β-萘黄酮导致肝脏的下调和g的上调，而在肠道中则呈上升趋势。ARNT是多种蛋白质的二聚体伴侣，包括介导对缺氧反应的缺氧诱导因子1α（HIF1α）。在暴露于β-萘黄酮的白st鱼肝脏中，HIF1α转录物的丰度下降。
更新日期：2020-02-20
中文翻译：
白色st鱼（Acipenser transmontanus）的芳烃受体核转运子（ARNT1，ARNT2和ARNT3）：序列，组织特异性表达和对β-萘黄酮的反应。
urge鱼（Acipenseridae）是古老的鱼类，具有对二恶英样化合物（DLCs）的转录反应的组织特异性特征，这与硬骨鱼类中的一般特征不同。由于DLC通过激活芳烃受体（AHR）发挥其关键毒性，因此该转录因子已成为深入研究的主题。但是，较少的注意力集中在芳烃受体核转运子（ARNT）上，它是AHR的二聚体伴侣，是AHR介导的转录所必需的。本研究对代表性a鱼，白色st鱼（Acipenser transmontanus）中的ARNT1，ARNT2和ARNT3进行了测序，并对每个ARNT的组织特异性基础转录本丰度和暴露于AHR模型激动剂后的响应进行了定量分析，β-萘黄酮。与st鱼中的其他蛋白质相同，ARNTs的氨基酸序列与四足动物的氨基酸序列比其他鱼类的ARNTs更为相似。在所有调查的组织中均检测到ARNT1，ARNT2和ARNT3的转录本。在脊椎动物中，ARNTs的表达受到严格的调节，但是β-萘黄酮导致肝脏的下调和g的上调，而在肠道中则呈上升趋势。ARNT是多种蛋白质的二聚体伴侣，包括介导对缺氧反应的缺氧诱导因子1α（HIF1α）。在暴露于β-萘黄酮的白st鱼肝脏中，HIF1α转录物的丰度下降。
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