Abstract Pentachlorophenol (PCP) is a toxic compound widely distributed in the environment. Rhizopus oryzae ENHE has been shown to possess PCP degradation capacities, peroxidases and phenoloxidases are expected to be involved in the degradation process but no studies have been performed that confirm this hypothesis. We have carried out a proteomic study aiming to understand the mechanisms by which R. oryze tolerates and degrades PCP. After culturing the fungus in media with and without PCP, the secretome and the intracellular proteome were analyzed by shotgun proteomics and 2D-Gel Electrophoresis, respectively. Proteins were identified by LC-MS/MS. In the intracellular proteome, 37 proteins were identified from the spots showing higher intensity or exclusive presence in cultures with PCP. Some redundant identifications of proteins appearing in more than one spot, like proteins belonging to energy metabolism, stress response and cell signaling. Proteins putatively involved in PCP degradation, like peroxidases and a methyltransferase, were also identified. In the secretome, 14 proteins were detected that showed at least 2-fold higher abundance in the broths from cultures with PCP. This work provides new information on the response of R. oryzae to the presence of PCP and allowed the identification of some proteins putatively involved in the degradation of the compound.

中文翻译：

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米根霉 ENHE 对五氯苯酚反应的蛋白质组学分析：了解这种有毒化合物的耐受和降解机制

摘要 五氯苯酚（PCP）是一种广泛分布于环境中的有毒化合物。米根霉 ENHE 已被证明具有 PCP 降解能力，预计过氧化物酶和酚氧化酶参与降解过程，但尚未进行任何研究证实这一假设。我们开展了一项蛋白质组学研究，旨在了解 R. oryze 耐受和降解 PCP 的机制。在含有和不含 PCP 的培养基中培养真菌后，分别通过鸟枪蛋白质组学和二维凝胶电泳分析分泌组和细胞内蛋白质组。通过LC-MS/MS鉴定蛋白质。在细胞内蛋白质组中，从带有 PCP 的培养物中显示出更高强度或唯一存在的点鉴定了 37 种蛋白质。出现在多个位置的蛋白质的一些冗余标识，例如属于能量代谢、应激反应和细胞信号传导的蛋白质。还鉴定了可能参与 PCP 降解的蛋白质，如过氧化物酶和甲基转移酶。在分泌组中，检测到 14 种蛋白质，它们在来自 PCP 培养物的肉汤中的丰度至少高出 2 倍。这项工作提供了关于米根霉对 PCP 存在的反应的新信息，并允许鉴定一些可能参与化合物降解的蛋白质。检测到 14 种蛋白质，它们在来自含有 PCP 的培养物的肉汤中的丰度至少高出 2 倍。这项工作提供了关于米根霉对 PCP 存在的反应的新信息，并允许鉴定一些可能参与化合物降解的蛋白质。检测到 14 种蛋白质，它们在来自含有 PCP 的培养物的肉汤中的丰度至少高出 2 倍。这项工作提供了关于米根霉对 PCP 存在的反应的新信息，并允许鉴定一些可能参与化合物降解的蛋白质。