Abstract Phase contrast microscopy enables the label-free visualization of “phase-only” objects such as unstained biological cells. This approach produces a contrast in image intensity that relates qualitatively to the phase delay introduced by the specimen. Quantitative phase imaging refers to an emerging set of methods that naturally extend phase contrast microscopy. These methods enable the quantitative measurement of the phase delay introduced by the specimen, which facilitates real-time measurement of cellular morphology with nanometric resolution in the axial direction. In this paper, we describe a simple, efficient algorithm that produces a color image from the quantitative phase image, which clearly highlights subcellular features. The approach is interpreted in terms of local spatial-frequency with the aid of microlens array and results are presented for a range of diatom cells.

中文翻译：

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定量相位图像的无标记颜色染色

摘要 相差显微镜使“仅相位”物体（例如未染色的生物细胞）的无标记可视化成为可能。这种方法产生图像强度的对比，定性地与样品引入的相位延迟相关。定量相位成像是指一组新兴的方法，可以自然地扩展相差显微镜。这些方法能够对样品引入的相位延迟进行定量测量，这有助于在轴向上以纳米分辨率实时测量细胞形态。在本文中，我们描述了一种简单、高效的算法，该算法从定量相位图像中生成彩色图像，清晰地突出了亚细胞特征。