The benchmark of this study is to evaluate the radio protective efficiency of diosmin, a natural citrus flavone of hesperidin derivative on radiation-induced damage in wistar albino rats. Rats orally administered two diosmin doses (100 and 200 mg/kg body wt.) for a month (every other day) prior to exposure to high gamma radiation single dose (8Gy) or cumulative dose (10Gy). To evaluate the radio protective efficiency of diosmin various biochemical estimations, histopathological alterations as well as comet assay and caspase-3 activity for assessment of apoptosis were performed. Results indicated that radiation-induced decline in the levels of antioxidant parameters (SOD and GSH), increased lipid peroxidation, DNA damage and apoptosis were improved by pre-administration of diosmin. Diosmin dose (200 mg/kg body wt.) restored the antioxidant status to near normal and reduced lipid peroxidation, DNA and tissue damage. These results were confirmed by histopathological examinations, which showed that pre-administration of diosmin protected the liver and kidney of albino rats against gamma-irradiation induced damage. Hence, it has been illustrated that diosmin might be an effective radio protector against radiation-induced damage in rats. Moreover, diosmin alone pretreated group did not show any biochemical alterations or DNA damage indicating the protective nature of the drug.

中文翻译：

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洋地黄在Wistar白化病大鼠中对抗由γ辐射引起的氧化应激诱导的损伤的保护作用。

这项研究的基准是评估地高辛（橙皮苷衍生物的天然柑橘类黄酮）对Wistar白化病大鼠辐射诱发的损伤的放射防护效率。大鼠在暴露于高伽马射线单剂量（8Gy）或累积剂量（10Gy）之前的一个月（隔日一次）内口服施用两次薯di素剂量（100和200 mg / kg体重）。为了评估薯os皂素的放射防护效率，进行了各种生化评估，组织病理学改变以及彗星试验和caspase-3活性来评估细胞凋亡。结果表明，通过预先加入薯os皂甙可以改善辐射引起的抗氧化剂参数（SOD和GSH）水平下降，脂质过氧化增加，DNA损伤和细胞凋亡。薯os素剂量（200 mg / kg体重 ）将抗氧化剂状态恢复到接近正常水平，并减少了脂质过氧化，DNA和组织损伤。这些结果由组织病理学检查证实，该结果表明，预先施用薯di皂甙可以保护白化病大鼠的肝脏和肾脏免受伽马射线辐照引起的损伤。因此，已经表明，薯os皂素可能是一种有效的辐射保护剂，可抵抗大鼠的辐射诱导损伤。此外，仅用薯os皂素预处理的组未显示任何生化改变或DNA损伤，表明该药物具有保护性。已经证明，薯os皂素可能是一种有效的辐射防护剂，可防止大鼠受到辐射诱发的损害。此外，仅用薯os皂素预处理的组未显示任何生化改变或DNA损伤，表明该药物具有保护性。已经证明，薯os皂素可能是一种有效的辐射防护剂，可防止大鼠受到辐射诱发的损害。此外，仅用薯os皂素预处理的组未显示任何生化改变或DNA损伤，表明该药物具有保护性。