Bio-orthogonal catalysis has the capability of localized generation of imaging and therapeutic molecules in vitro and in vivo. The integration of these catalysts into thermoresponsive nanoparticle platforms would generate bio-orthogonal “nanozymes” that could be controlled through endogenous or exogenous thermal control. We have fabricated thermoresponsive nanozymes by confining supramolecular assemblies of porphyrins into the monolayer of gold nanoparticles. The resulting nanodevices feature an on-off gated thermal response occurring over a 3°C range with commensurate tunability of activation temperature from 25°C to 37°C. Reversible activation of catalysis was demonstrated in complex biological environments, and the efficacy of bi-stable thermoresponsive nanozymes demonstrated through thermal activation of antibiotic-based prodrugs to effectively treat bacterial biofilms.

生物正交催化具有在体外和体内局部生成成像和治疗分子的能力。将这些催化剂整合到热响应性纳米颗粒平台中会产生生物正交的“纳米酶”，可以通过内源性或外源性热控制进行控制。我们通过将卟啉的超分子组装限制在金纳米颗粒的单层中来制造热响应性纳米酶。所得的纳米器件具有在3°C的范围内发生开-关门热响应的特性，并且激活温度从25°C到37°C具有相称的可调性。在复杂的生物环境中证明了催化的可逆激活，并且通过对基于抗生素的前药进行热激活来有效处理细菌生物膜，证明了双稳态热响应纳米酶的功效。