A Well-Mixed E. coli Genome: Widespread Contacts Revealed by Tracking Mu Transposition.,Cell

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A Well-Mixed E. coli Genome: Widespread Contacts Revealed by Tracking Mu Transposition.
Cell ( IF 45.5 ) Pub Date : 2020-02-10 , DOI: 10.1016/j.cell.2020.01.031
David M Walker ₁ , Peter L Freddolino ₂ , Rasika M Harshey ₁

Affiliation

The three-dimensional structures of chromosomes are increasingly being recognized as playing a major role in cellular regulatory states. The efficiency and promiscuity of phage Mu transposition was exploited to directly measure in vivo interactions between genomic loci in E. coli. Two global organizing principles have emerged: first, the chromosome is well-mixed and uncompartmentalized, with transpositions occurring freely between all measured loci; second, several gene families/regions show "clustering": strong three-dimensional co-localization regardless of linear genomic distance. The activities of the SMC/condensin protein MukB and nucleoid-compacting protein subunit HU-α are essential for the well-mixed state; HU-α is also needed for clustering of 6/7 ribosomal RNA-encoding loci. The data are explained by a model in which the chromosomal structure is driven by dynamic competition between DNA replication and chromosomal relaxation, providing a foundation for determining how region-specific properties contribute to both chromosomal structure and gene regulation.

中文翻译：

充分混合的大肠杆菌基因组：通过追踪Mu换位揭示了广泛的接触。

越来越多地认识到染色体的三维结构在细胞调节状态中起着重要作用。利用噬菌体Mu转座的效率和混杂性来直接测量大肠杆菌中基因组基因座之间的体内相互作用。已经出现了两种全球组织原则：首先，染色体是充分混合且没有隔室的，在所有测得的基因座之间自由发生转座。第二，几个基因家族/区域表现出“聚类”：无论线性基因组距离如何，强三维共定位。SMC /凝缩蛋白MukB和类紧缩蛋白亚基HU-α的活性对于充分混合状态是必不可少的。HU-α对于6/7核糖体RNA编码基因座的聚类也是必需的。

更新日期：2020-02-20

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